Neuroprotective phenylpropanoid esters of rhamnose isolated from roots of Scrophularia buergeriana

• Published in: Phytochemistry (Oxford) Vol. 54; no. 5; pp. 503 - 509
• Main Authors: Kim, So Ra, Kim, Young Choong
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.06.2000; Elsevier
• Subjects: Animals; Biochemistry & Molecular Biology; Biological and medical sciences; Buergerisides; Cell Survival - drug effects; Cells, Cultured; Cerebral Cortex - cytology; Chemical constitution; Excitotoxicity; Fetus; Fundamental and applied biological sciences. Psychology; General pharmacology; Glycosides - chemistry; Glycosides - isolation & purification; Glycosides - pharmacology; L-glutamate; Life Sciences & Biomedicine; Medical sciences; Molecular Structure; Neuroglia - cytology; Neuroglia - drug effects; Neurons - cytology; Neurons - drug effects; Neuroprotective activity; Neuroprotective Agents - chemistry; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenylpropanoids; Plant physiology and development; Plant Roots - chemistry; Plant Sciences; Plants, Medicinal - chemistry; Primary cultures of rat cortical cells; Rats; Rats, Sprague-Dawley; Rhamnose - analogs & derivatives; Science & Technology; Scrophularia buergeriana; Scrophulariaceae; Primary cultures of rat cortical cells; Excitotoxicity; Scrophularia buergeriana; Scrophulariaceae; Buergerisides; Neuroprotective activity; Phenylpropanoids; L-glutamate; CORTICAL CELL-CULTURE; NERVOUS-SYSTEM; GLUTAMATE NEUROTOXICITY; IRIDOID GLYCOSIDES; primary cultures of rat cortical cells; SCOPOLII; BRAIN ISCHEMIA; excitotoxicity; phenylpropanoids; neuroprotective activity; NINGPOENSIS; Root; Molecular structure; Cinnamic acid; Neuroprotective agent; In vitro; Biological activity; Dose activity relation; Ester; Dicotyledones; Angiospermae; Plant origin; Rhamnose; Isolation; Spermatophyta
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(00)00110-2

Four phenylpropanoid esters of rhamnose, buergerisides A 1, B 1, B 2 and C 1 were isolated from roots of Scrophularia buergeriana MIQ. (Scrophulariaceae), and were characterized as 2- O-acetyl-3,4-di- O-( E)- p-methoxycinnamoyl-α- L-rhamnopyranoside, 2- O-acetyl-3- O-( E)- p-methoxycinnamoyl-α- L-rhamnopyranoside, 2- O-acetyl-3- O-( Z)- p-methoxycinnamoyl-α- L-rhamnopyranoside and 4- O-( E)- p-methoxycinnamoyl-α- L-rhamnopyranoside, respectively. In addition, six known phenylpropanoids were authenticated as: ( E)-cinnamic acid, ( E)- p-methoxycinnamic acid, ( E)- p-methoxycinnamic acid methyl ester, ( E)- p-coumaric acid, ( E)-caffeic acid, ( E)-ferulic acid and a phenylalcohol, 2-(3-hydroxy-4-methoxyphenyl)ethanol. These ten phenylpropanoids all attenuated glutamate-induced neurotoxicity when added to primary cultures of rat cortical cells in a dose-dependent manner. These results demonstrate that phenylpropanoids isolated from S. buergeriana may exert significant protective effects against glutamate-induced neurodegeneration in primary cultures of cortical neurons.