The microscale evolution of the erosion front of blood clots exposed to ultrasound stimulated microbubbles

Serial two-photon microscopy of blood clots with fluorescently tagged fibrin networks was conducted during microbubble-mediated sonothrombolysis to examine the microscale evolution of the resulting erosion front. The development of a complex zonal erosion pattern was observed, comprised of a cell de...

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Bibliographic Details
Published inThe Journal of the Acoustical Society of America Vol. 139; no. 5; pp. EL135 - EL141
Main Authors Acconcia, Christopher N., Leung, Ben Y. C., Goertz, David E.
Format Journal Article
LanguageEnglish
Published United States 01.05.2016
Subjects
Erythrocytes - metabolism
Fibrin - metabolism
Fibrinolysis
Humans
Mechanical Thrombolysis - methods
Microbubbles
Microscopy, Fluorescence, Multiphoton
Thrombosis - blood
Thrombosis - therapy
Time Factors
Ultrasonic Therapy - methods
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Summary:Serial two-photon microscopy of blood clots with fluorescently tagged fibrin networks was conducted during microbubble-mediated sonothrombolysis to examine the microscale evolution of the resulting erosion front. The development of a complex zonal erosion pattern was observed, comprised of a cell depleted layer of fibrin network overlying intact clot which then underwent progressive recession. The fibrin zone architecture was dependent on exposure conditions with 0.1 MPa causing no erosion, 0.39 MPa resulting in homogenous structure, and combination 0.39/0.96 MPa pulses forming large-scale tunnels. High speed imaging and Coulter counter data indicated the fibrin zone formation process involves the ejection of intact erythrocytes.
ISSN:0001-4966
1520-8524
DOI:10.1121/1.4946045