Short Exposure of Polymorphonuclear Leucocytes to Sodium Fluoride Suppresses the Response to fMLP

• Published in: Journal of trace elements in medicine and biology Vol. 13; no. 1; pp. 68 - 75
• Main Authors: Knoll-Köhler, E., Büssem, G.
• Format: Journal Article
• Language: English
• Published: Stuttgart Elsevier GmbH 01.07.1999; Fischer
• Subjects: [Ca 2+]i; Adult; Biological and medical sciences; Calcium - blood; cAMP; Cyclic AMP - blood; dentistry; Exocytosis; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; fluoride; Humans; Hypochlorous Acid - blood; Hypochlorous Acid - chemistry; Kinetics; Lysosomes - enzymology; Male; Medical sciences; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophils - drug effects; Neutrophils - physiology; Non tumoral diseases; O 2; Otorhinolaryngology. Stomatology; polymorphonuclear leucocytes; Respiratory Burst; Saliva - chemistry; Sodium Fluoride - administration & dosage; Sodium Fluoride - pharmacology; Superoxides - blood; Taurine - chemistry; [Ca 2+]i; O 2; P; fMLP; dentistry; polymorphonuclear leucocytes; IBMX; PMNs; fluoride; cAMP; Ca 2; Human; Calcium ion; Enzyme; Leukocyte; Granulocyte; Lysosome; Biological effect; Sodium fluoride; Saliva; Bactericidal effect
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/S0946-672X(99)80026-1

Fluoridated dental care products are used to prevent dental decay. Up to now, there are no data available on whether the fluoride (F -) component of these products affects the bactericidal activity of salivary polymorphonuclear leucocytes, which are involved in the protection of the oral mucosa against infection. Therefore, after determining the concentration/time profile of F - in mixed saliva of healthy subjects after topical application of 0.5 g of a 1.25 % F - containing gel, unstimulated and fMLP-stimulated polymorphonuclear leucocytes (PMNs) were shortly exposed to these F - concentrations and the generation of Superoxide and hypochloric acid were measured, as well as the liberation of lysomal enzymes, and correlated with the cellular Ca 2+and cAMP-levels. The results show that F -, at concentrations as retained in saliva, did not activate the oxidative burst in unstimulated PMNs. In fMLP-activated PMNs, F - suppressed the receptor-mediated increase in the oxidative burst and the liberation of fl-glucuronidase by reduction of the availability of extracellular Ca 2+ and, thus, the influx of Ca 2+ necessary to couple completely the fMLP signal to effector pathways. These F - concentrations neither altered the liberation of Ca 2+ from internal stores nor induced a rise in cAMP. The possible clinical consequences of these results for xerostomic patients with respect to the generation of HOSCN/OSCN/SCN - in saliva, an important non-immune factor for oral health, are dicussed.