Chemical synthesis of gamma-echistatin analogues: elucidation of status of C-terminal (45-49)

Three analogues of gamma-echistatin, des(45-49)-gamma-echistatin, des(46-49)-gamma-echistatin and des(47-49)-gamma-echistatin, were synthesized by solid-phase methodology and their biological activities were measured and compared. The results reveal that without the C-terminal (45-49) of gamma-echis...

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Bibliographic Details
Published inProtein engineering Vol. 7; no. 7; p. 941
Main Authors Chen, P Y, Wu, S H, Wang, K T
Format Journal Article
LanguageEnglish
Published England 01.07.1994
Subjects
Amino Acid Sequence
Circular Dichroism
Mass Spectrometry
Molecular Sequence Data
Oxidation-Reduction
Peptide Fragments - chemical synthesis
Peptide Fragments - chemistry
Peptide Fragments - pharmacology
Peptides
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - pharmacology
Protein Conformation
Protein Folding
Sequence Alignment
Structure-Activity Relationship
Viper Venoms - chemistry
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Summary:Three analogues of gamma-echistatin, des(45-49)-gamma-echistatin, des(46-49)-gamma-echistatin and des(47-49)-gamma-echistatin, were synthesized by solid-phase methodology and their biological activities were measured and compared. The results reveal that without the C-terminal (45-49) of gamma-echistatin, the folding of the protein to the final active structure is not interfered with and Lys-45 influences the inhibition of platelet aggregation.
ISSN:0269-2139
DOI:10.1093/protein/7.7.941