First derivative synchronous fluorescence spectroscopy for the determination of Gatifloxacin in presence of its oxidative degradation product: Application to pharmaceutical preparation

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 206; pp. 302 - 313
• Main Authors: Salama, Fathy M.M., Attia, Khalid A.M., Said, Ragab A.M., El-Attar, Abdul-Aziz M.M.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.01.2019
• Subjects: Antimicrobial susceptibility; Bacteria - drug effects; Drug Stability; Gatifloxacin; Gatifloxacin - analysis; Gatifloxacin - chemistry; Gatifloxacin - pharmacology; Limit of Detection; Linear Models; Micelle enhanced fluorescence; Microbial Sensitivity Tests; Numerical differentiation; Oxidation-Reduction; Pharmaceutical preparations; Spectrometry, Fluorescence - methods; Synchronous fluorimetry; Tablets; Synchronous fluorimetry; Pharmaceutical preparations; Antimicrobial susceptibility; Micelle enhanced fluorescence; Gatifloxacin; Numerical differentiation
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2018.08.003

A highly accurate and precise spectrofluorimetric method was established for quantitation of Gatifloxacin in pure material, pharmaceutical formulations and in the existence of its oxidative degradation product. The emission was recorded at 487 nm after the excitation at 290 nm. Using micelle, sodium dodecyl sulphate (SDS), enhanced fluorescence intensity of Gatifloxacin-SDS complex. The optimization of numerous experimental conditions was carried out. The improved emission showed a suitable linear correlation between derivative synchronous fluorescence power and concentration of Gatifloxacin over the range of 10 to 100 ng/mL with a determination coefficient equals 0.9996. Studying cytotoxicity and antimicrobial susceptibility for oxidative degradation product of Gatifloxacin was carried out using Gatifloxacin as a control. In comparison, the proposed method presented a superior sensitivity and enhanced stability over the reported method. [Display omitted] •Confirmation of Gatifloxacin degradation•Micelle, SDS, can improve the fluorescence intensity.•Solving the severely overlapped spectra•Antimicrobial and toxic effect of oxidative degradation product•Analysis of the pharmaceutical dosage form without the inference of excipients.