Use of hyperspectral imaging for label-free decoding and detection of biomarkers

Detecting the existence of biomarkers is one of the most important issues in molecular diagnosis. This Letter presents a label-free decoding and detection method for biomarker tests based on hyperspectral imaging and interferometry. The decoding and detection results were extracted from a single hyp...

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Bibliographic Details
Published inOptics letters Vol. 38; no. 9; p. 1524
Main Authors Wang, Tongzhou, Li, Qiang, Li, Xin, Zhao, Songmin, Lu, Ying, Huang, Guoliang
Format Journal Article
LanguageEnglish
Published United States 01.05.2013
Subjects
Animals
Biomarkers - blood
Humans
Immunoglobulin G - blood
Interferometry
Molecular Imaging - methods
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Summary:Detecting the existence of biomarkers is one of the most important issues in molecular diagnosis. This Letter presents a label-free decoding and detection method for biomarker tests based on hyperspectral imaging and interferometry. The decoding and detection results were extracted from a single hyperspectral image with different spectrum-to-thickness reconstructing algorithms, which made the testing procedure extremely fast and simple. The coding capacity of this method is more than 400, and the detection sensitivity can reach 2 ng/mm2 without fluorescent labeling.
ISSN:1539-4794
DOI:10.1364/ol.38.001524