Plasmid diversity among genetically related Klebsiella pneumoniae blaKPC-2 and blaKPC-3 isolates collected in the Dutch national surveillance

Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and d...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 10; no. 1; p. 16778
Main Authors Hendrickx, Antoni P. A., Landman, Fabian, de Haan, Angela, Borst, Dyogo, Witteveen, Sandra, van Santen-Verheuvel, Marga G., van der Heide, Han G. J., Schouls, Leo M.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 08.10.2020
Subjects
631/114
631/326
Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
Online AccessGet full text

Cover

Abstract Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and diversity of the plasmids of genetically related K. pneumoniae strains harboring the beta-lactamase gene bla KPC-2 or bla KPC-3 to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014 to 2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 22 plasmids. wgMLST revealed five genetic clusters comprised of K. pneumoniae bla KPC-2 isolates and four clusters consisted of bla KPC-3 isolates. KpnCluster-019 bla KPC-2 isolates were found both in the Netherlands and the Caribbean islands, while bla KPC-3 cluster isolates only in the Netherlands. Each K. pneumoniae bla KPC-2 or bla KPC-3 cluster was characterized by a distinct resistome and plasmidome. However, the large and medium plasmids contained a variety of antibiotic resistance genes, conjugation machinery, cation transport systems, transposons, toxin/antitoxins, insertion sequences and prophage-related elements. The small plasmids carried genes implicated in virulence. Thus, implementing long-read plasmid sequencing analysis for K. pneumoniae surveillance provided important insights in the transmission of a KpnCluster-019 bla KPC-2 strain between the Netherlands and the Caribbean.
AbstractList Abstract Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and diversity of the plasmids of genetically related K. pneumoniae strains harboring the beta-lactamase gene bla KPC-2 or bla KPC-3 to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014 to 2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 22 plasmids. wgMLST revealed five genetic clusters comprised of K. pneumoniae bla KPC-2 isolates and four clusters consisted of bla KPC-3 isolates. KpnCluster-019 bla KPC-2 isolates were found both in the Netherlands and the Caribbean islands, while bla KPC-3 cluster isolates only in the Netherlands. Each K. pneumoniae bla KPC-2 or bla KPC-3 cluster was characterized by a distinct resistome and plasmidome. However, the large and medium plasmids contained a variety of antibiotic resistance genes, conjugation machinery, cation transport systems, transposons, toxin/antitoxins, insertion sequences and prophage-related elements. The small plasmids carried genes implicated in virulence. Thus, implementing long-read plasmid sequencing analysis for K. pneumoniae surveillance provided important insights in the transmission of a KpnCluster-019 bla KPC-2 strain between the Netherlands and the Caribbean.
Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is important to track the spread of K. pneumoniae and its plasmids between patients. Therefore, the major aim was to recapitulate the contents and diversity of the plasmids of genetically related K. pneumoniae strains harboring the beta-lactamase gene bla KPC-2 or bla KPC-3 to determine their dissemination in the Netherlands and the former Dutch Caribbean islands from 2014 to 2019. Next-generation sequencing was combined with long-read third-generation sequencing to reconstruct 22 plasmids. wgMLST revealed five genetic clusters comprised of K. pneumoniae bla KPC-2 isolates and four clusters consisted of bla KPC-3 isolates. KpnCluster-019 bla KPC-2 isolates were found both in the Netherlands and the Caribbean islands, while bla KPC-3 cluster isolates only in the Netherlands. Each K. pneumoniae bla KPC-2 or bla KPC-3 cluster was characterized by a distinct resistome and plasmidome. However, the large and medium plasmids contained a variety of antibiotic resistance genes, conjugation machinery, cation transport systems, transposons, toxin/antitoxins, insertion sequences and prophage-related elements. The small plasmids carried genes implicated in virulence. Thus, implementing long-read plasmid sequencing analysis for K. pneumoniae surveillance provided important insights in the transmission of a KpnCluster-019 bla KPC-2 strain between the Netherlands and the Caribbean.
ArticleNumber 16778
Author Schouls, Leo M.
Witteveen, Sandra
Hendrickx, Antoni P. A.
van Santen-Verheuvel, Marga G.
de Haan, Angela
Borst, Dyogo
Landman, Fabian
van der Heide, Han G. J.
Author_xml – sequence: 1
  givenname: Antoni P. A.
  surname: Hendrickx
  fullname: Hendrickx, Antoni P. A.
  email: antoni.hendrickx@rivm.nl
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 2
  givenname: Fabian
  surname: Landman
  fullname: Landman, Fabian
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 3
  givenname: Angela
  surname: de Haan
  fullname: de Haan, Angela
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 4
  givenname: Dyogo
  surname: Borst
  fullname: Borst, Dyogo
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 5
  givenname: Sandra
  surname: Witteveen
  fullname: Witteveen, Sandra
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 6
  givenname: Marga G.
  surname: van Santen-Verheuvel
  fullname: van Santen-Verheuvel, Marga G.
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 7
  givenname: Han G. J.
  surname: van der Heide
  fullname: van der Heide, Han G. J.
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
– sequence: 8
  givenname: Leo M.
  surname: Schouls
  fullname: Schouls, Leo M.
  organization: Center for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM)
BookMark eNp9kc9u1DAQxi1UREvpC3DykUvA8Z8kviChBVrUSvTQuzVxZnddOfZiOyvtQ_Sd8bIFwYW5eCx_v2_k-V6TsxADEvK2Ze9bJoYPWbZKDw3jrOmFlKzhL8gFZ1I1XHB-9ld_Tq5yfmS1FNey1a_IuRBMCK7FBXm695BnN9HJ7TFlVw4U5hg2dIMBi7Pg_YEm9FBworcex-zQe6C7gEvVOUA6eri9XzWcQph-XwR1OR6hTG30Hu0Rd4GWLdLPS7FbGqC4GMDTvKQ9uuoZLL4hL9fgM149n5fk4euXh9VNc_f9-tvq011jhWalkahASqXrH_ikuQKtBrRjr8Eybqeuw5EzWLOOQd_rcey00B2XoHomh2kQl-TjyXa3jDNOFkNJ4M0uuRnSwURw5t-X4LZmE_emV7LrWl0N3j0bpPhjwVzM7LI9LiZgXLLhUmqttOxFlfKT1KaYc8L1nzEtM8ckzSlJU5M0v5I0vELiBOUqDhtM5jEuqW4r_4_6CasPo6I
CitedBy_id crossref_primary_10_1038_s41598_021_95285_z
crossref_primary_10_1089_mdr_2022_0143
crossref_primary_10_1093_jacamr_dlac041
crossref_primary_10_1099_mgen_0_000512
crossref_primary_10_1080_20477724_2022_2121362
crossref_primary_10_1038_s43856_022_00200_w
crossref_primary_10_1099_mgen_0_001026
crossref_primary_10_1128_aac_00860_23
crossref_primary_10_1016_j_jwpe_2023_104732
crossref_primary_10_1016_j_jgar_2022_09_006
crossref_primary_10_1186_s12864_021_08279_6
crossref_primary_10_1128_spectrum_03395_22
crossref_primary_10_3389_fmicb_2021_622280
crossref_primary_10_1128_mBio_01013_21
crossref_primary_10_1128_spectrum_02564_23
crossref_primary_10_3389_fmicb_2022_866674
crossref_primary_10_1016_j_celrep_2024_114351
crossref_primary_10_1038_s41598_021_00887_2
crossref_primary_10_1038_s43856_022_00115_6
crossref_primary_10_1371_journal_pgen_1009919
crossref_primary_10_1093_jacamr_dlab046
Cites_doi 10.1016/j.tim.2016.09.007
10.1128/JCM.43.8.4178-4182.2005
10.1128/mBio.00489-17
10.1371/journal.pcbi.1005595
10.1111/nyas.14223
10.1093/jac/dkx488
10.1128/AAC.05202-11
10.1038/nmeth.3930
10.1371/journal.pgen.1008114
10.1128/AAC.02412-14
10.1089/mdr.2015.0083
10.1128/AAC.05308-11
10.1128/CMR.00116-14
10.1093/femsre/fux013
10.1093/cid/cix893
10.1042/bj2760269
10.1093/nar/gkw387
10.1128/AAC.01451-07
10.1128/JCM.00535-17
10.1093/bioinformatics/btu555
10.1128/CMR.0001-19
10.1128/AAC.03183-14
10.1016/j.cmi.2020.01.027
10.1016/j.mib.2018.04.004
10.1093/bioinformatics/btu153
10.1016/j.jgar.2018.11.017
10.3389/fmicb.2019.00542
10.1016/j.gpb.2016.05.004
10.1128/AAC.00076-17
10.1038/s41598-019-54895-4
10.1128/AAC.02534-16
10.1093/jac/dkm226
10.1073/pnas.1501049112
10.1371/journal.pone.0123690
10.1016/j.cmi.2017.01.015
10.1128/AAC.49.11.4760-4762.2005
10.1080/21505594.2016.1222343
10.1080/21505594.2017.1292196
10.1016/S1473-3099(13)70190-7
10.1093/jac/dks261
10.1556/030.65.2018.031
ContentType Journal Article
Copyright The Author(s) 2020
Copyright_xml – notice: The Author(s) 2020
CorporateAuthor The Dutch CPE surveillance Study Group
CorporateAuthor_xml – name: The Dutch CPE surveillance Study Group
DBID C6C
AAYXX
CITATION
7X8
5PM
DOI 10.1038/s41598-020-73440-2
DatabaseName Springer_OA刊
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle CrossRef
MEDLINE - Academic
DatabaseTitleList CrossRef
Database_xml – sequence: 1
  dbid: C6C
  name: Springer_OA刊
  url: http://www.springeropen.com/
  sourceTypes: Publisher
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2045-2322
EndPage 16778
ExternalDocumentID 10_1038_s41598_020_73440_2
GroupedDBID 0R~
3V.
4.4
53G
5VS
7X7
88A
88E
88I
8FE
8FH
8FI
8FJ
AAFWJ
AAJSJ
AAKDD
ABDBF
ABUWG
ACGFS
ACSMW
ADBBV
ADRAZ
AENEX
AFKRA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
DWQXO
EBD
EBLON
EBS
ESX
FYUFA
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
KQ8
LK8
M0L
M1P
M2P
M48
M7P
M~E
NAO
OK1
PIMPY
PQQKQ
PROAC
PSQYO
RIG
RNT
RNTTT
RPM
SNYQT
UKHRP
AAYXX
AFPKN
CITATION
7X8
5PM
ID FETCH-LOGICAL-c390t-4e5a44593322d925a958ecb79ac02cd66eb20af060a779bb6939624a57048d83
IEDL.DBID RPM
ISSN 2045-2322
IngestDate Tue Sep 17 21:24:28 EDT 2024
Sat Oct 05 04:36:50 EDT 2024
Fri Aug 23 01:56:29 EDT 2024
Fri Oct 11 20:49:36 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c390t-4e5a44593322d925a958ecb79ac02cd66eb20af060a779bb6939624a57048d83
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546619/
PMID 33033293
PQID 2449959473
PQPubID 23479
PageCount 1
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_7546619
proquest_miscellaneous_2449959473
crossref_primary_10_1038_s41598_020_73440_2
springer_journals_10_1038_s41598_020_73440_2
PublicationCentury 2000
PublicationDate 2020-10-08
PublicationDateYYYYMMDD 2020-10-08
PublicationDate_xml – month: 10
  year: 2020
  text: 2020-10-08
  day: 08
PublicationDecade 2020
PublicationPlace London
PublicationPlace_xml – name: London
PublicationTitle Scientific reports
PublicationTitleAbbrev Sci Rep
PublicationYear 2020
Publisher Nature Publishing Group UK
Publisher_xml – name: Nature Publishing Group UK
References Kopotsa, Osei Sekyere, Mbelle (CR2) 2019
Billard-Pomares (CR31) 2014; 58
Bosch (CR16) 2017; 55
García-Fernández (CR45) 2012; 56
Wyres, Holt (CR7) 2016; 24
Seemann (CR40) 2014; 30
Alba, Ishii, Thomson, Moland, Yamaguchi (CR15) 2005; 49
van Duin, Doi (CR1) 2017; 8
Trecarichi, Tumbarello (CR4) 2017; 8
Carattoli (CR35) 2014; 58
Wesley Long (CR42) 2017; 8
Mathers, Peirano, Pitout (CR13) 2015; 28
Holt (CR23) 2015; 112
van der Zwaluw (CR33) 2015; 10
Arndt (CR41) 2016; 44
Navon-Venezia, Kondratyeva, Carattoli (CR6) 2017; 41
Huang (CR25) 2017
Loose, Malla, Stout (CR37) 2016; 13
Loman, Quinlan (CR38) 2014; 30
Wyres, Holt (CR5) 2018; 45
de Lannoy, de Ridder, Risse (CR19) 2017; 6
Munoz-Price (CR11) 2013; 13
Roe (CR43) 2019; 10
van der Zwaluw (CR21) 2020
Feng, Liu, McNally, Zong (CR26) 2019; 17
Hanczvikkel, Füzi, Ungvári, Tóth (CR30) 2018; 65
Walther-Rasmussen, Høiby (CR14) 2007; 60
Diancourt, Passet, Verhoef, Grimont, Brisse (CR34) 2005; 43
Boolchandani, D’Souza, Dantas (CR17) 2019; 20
Wyres (CR29) 2019; 15
Rozwandowicz (CR8) 2018; 73
Zankari (CR36) 2012; 67
Ambler (CR12) 1991; 276
Lu, Giordano, Ning (CR18) 2016; 14
Bonomo (CR3) 2018; 66
CR20
Naas (CR10) 2008; 52
Russo, Marr (CR24) 2019
Kocsis (CR28) 2016; 22
Ruppé (CR44) 2017; 237
Liang (CR22) 2019; 70
Haidar (CR27) 2017
Wick, Judd, Gorrie, Holt (CR39) 2017; 13
Cuzon, Naas, Nordmann (CR9) 2011; 55
Venturini (CR32) 2019; 9
M Rozwandowicz (73440_CR8) 2018; 73
RP Ambler (73440_CR12) 1991; 276
Y Feng (73440_CR26) 2019; 17
A García-Fernández (73440_CR45) 2012; 56
S Navon-Venezia (73440_CR6) 2017; 41
K van der Zwaluw (73440_CR21) 2020
KL Wyres (73440_CR29) 2019; 15
K Kopotsa (73440_CR2) 2019
L Diancourt (73440_CR34) 2005; 43
T Billard-Pomares (73440_CR31) 2014; 58
G Cuzon (73440_CR9) 2011; 55
M Boolchandani (73440_CR17) 2019; 20
G Haidar (73440_CR27) 2017
CC Roe (73440_CR43) 2019; 10
J Walther-Rasmussen (73440_CR14) 2007; 60
LS Munoz-Price (73440_CR11) 2013; 13
73440_CR20
E Kocsis (73440_CR28) 2016; 22
KL Wyres (73440_CR5) 2018; 45
T Bosch (73440_CR16) 2017; 55
A Carattoli (73440_CR35) 2014; 58
NJ Loman (73440_CR38) 2014; 30
E Zankari (73440_CR36) 2012; 67
M Loose (73440_CR37) 2016; 13
EM Trecarichi (73440_CR4) 2017; 8
J Alba (73440_CR15) 2005; 49
C de Lannoy (73440_CR19) 2017; 6
W Liang (73440_CR22) 2019; 70
KE Holt (73440_CR23) 2015; 112
A Hanczvikkel (73440_CR30) 2018; 65
RR Wick (73440_CR39) 2017; 13
K van der Zwaluw (73440_CR33) 2015; 10
T Seemann (73440_CR40) 2014; 30
TA Russo (73440_CR24) 2019
KL Wyres (73440_CR7) 2016; 24
H Lu (73440_CR18) 2016; 14
E Ruppé (73440_CR44) 2017; 237
C Venturini (73440_CR32) 2019; 9
AJ Mathers (73440_CR13) 2015; 28
W Huang (73440_CR25) 2017
D van Duin (73440_CR1) 2017; 8
RA Bonomo (73440_CR3) 2018; 66
T Naas (73440_CR10) 2008; 52
D Arndt (73440_CR41) 2016; 44
SW Wesley Long (73440_CR42) 2017; 8
References_xml – volume: 24
  start-page: 944
  year: 2016
  end-page: 956
  ident: CR7
  article-title: population genomics and antimicrobial-resistant clones
  publication-title: Trends Microbiol.
  doi: 10.1016/j.tim.2016.09.007
  contributor:
    fullname: Holt
– volume: 43
  start-page: 4178
  year: 2005
  end-page: 4182
  ident: CR34
  article-title: Multilocus sequence typing of nosocomial isolates
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.43.8.4178-4182.2005
  contributor:
    fullname: Brisse
– volume: 65
  start-page: 387
  year: 2018
  end-page: 403
  ident: CR30
  article-title: Transmissible silver resistance readily evolves in high-risk clone isolates of
  publication-title: Acta Microbiol. Immunol. Hung.
  contributor:
    fullname: Tóth
– volume: 20
  start-page: 356
  year: 2019
  end-page: 370
  ident: CR17
  article-title: Sequencing-based methods and resources to study antimicrobial resistance
  publication-title: Nat. Rev. Genet.
  contributor:
    fullname: Dantas
– volume: 8
  start-page: e00489-17
  issue: 3
  year: 2017
  ident: CR42
  article-title: Population genomic analysis of 1,777 extended-spectrum beta-lactamase-producing isolates, Houston, Texas: Unexpected abundance of clonal group 307
  publication-title: mBio
  doi: 10.1128/mBio.00489-17
  contributor:
    fullname: Wesley Long
– volume: 13
  start-page: e1005595
  year: 2017
  ident: CR39
  article-title: Unicycler: resolving bacterial genome assemblies from short and long sequencing reads
  publication-title: PLoS Comput. Biol.
  doi: 10.1371/journal.pcbi.1005595
  contributor:
    fullname: Holt
– year: 2019
  ident: CR2
  article-title: Plasmid evolution in carbapenemase-producing : a review
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1111/nyas.14223
  contributor:
    fullname: Mbelle
– volume: 73
  start-page: 1121
  year: 2018
  end-page: 1137
  ident: CR8
  article-title: Plasmids carrying antimicrobial resistance genes in
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dkx488
  contributor:
    fullname: Rozwandowicz
– volume: 55
  start-page: 5370
  year: 2011
  end-page: 5373
  ident: CR9
  article-title: Functional characterization of Tn , a Tn -based transposon involved in KPC gene mobilization
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.05202-11
  contributor:
    fullname: Nordmann
– volume: 70
  start-page: 84
  year: 2019
  end-page: 89
  ident: CR22
  article-title: genes are ubiquitous in clinical, KPC-2-positive, isolates
  publication-title: Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis.
  contributor:
    fullname: Liang
– volume: 6
  start-page: 1083
  year: 2017
  ident: CR19
  article-title: The long reads ahead: genome assembly using theMiniON
  publication-title: F1000Research
  contributor:
    fullname: Risse
– volume: 13
  start-page: 751
  year: 2016
  end-page: 754
  ident: CR37
  article-title: Real-time selective sequencing using nanopore technology
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3930
  contributor:
    fullname: Stout
– volume: 15
  start-page: e1008114
  year: 2019
  ident: CR29
  article-title: Distinct evolutionary dynamics of horizontal gene transfer in drug resistant and virulent clones of
  publication-title: PLoS Genet.
  doi: 10.1371/journal.pgen.1008114
  contributor:
    fullname: Wyres
– volume: 58
  start-page: 3895
  year: 2014
  end-page: 3903
  ident: CR35
  article-title: detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.02412-14
  contributor:
    fullname: Carattoli
– volume: 22
  start-page: 123
  year: 2016
  end-page: 128
  ident: CR28
  article-title: blaNDM-1 carriage on IncR plasmid in strains
  publication-title: Microb. Drug Resist.
  doi: 10.1089/mdr.2015.0083
  contributor:
    fullname: Kocsis
– volume: 56
  start-page: 2143
  year: 2012
  end-page: 2145
  ident: CR45
  article-title: ST258 producing KPC-3 identified in italy carries novel plasmids and OmpK36/OmpK35 porin variants
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.05308-11
  contributor:
    fullname: García-Fernández
– volume: 28
  start-page: 565
  year: 2015
  end-page: 591
  ident: CR13
  article-title: The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.00116-14
  contributor:
    fullname: Pitout
– volume: 41
  start-page: 252
  year: 2017
  end-page: 275
  ident: CR6
  article-title: : a major worldwide source and shuttle for antibiotic resistance
  publication-title: FEMS Microbiol. Rev.
  doi: 10.1093/femsre/fux013
  contributor:
    fullname: Carattoli
– volume: 66
  start-page: 1290
  year: 2018
  end-page: 1297
  ident: CR3
  article-title: Carbapenemase-producing organisms: a global scourge
  publication-title: Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am.
  doi: 10.1093/cid/cix893
  contributor:
    fullname: Bonomo
– volume: 276
  start-page: 269
  issue: Pt 1
  year: 1991
  end-page: 270
  ident: CR12
  article-title: A standard numbering scheme for the class A beta-lactamases
  publication-title: Biochem. J.
  doi: 10.1042/bj2760269
  contributor:
    fullname: Ambler
– volume: 44
  start-page: W16
  year: 2016
  end-page: 21
  ident: CR41
  article-title: PHASTER: a better, faster version of the PHAST phage search tool
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkw387
  contributor:
    fullname: Arndt
– volume: 52
  start-page: 1257
  year: 2008
  end-page: 1263
  ident: CR10
  article-title: Genetic structures at the origin of acquisition of the beta-lactamase KPC gene
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.01451-07
  contributor:
    fullname: Naas
– volume: 55
  start-page: 2380
  year: 2017
  end-page: 2390
  ident: CR16
  article-title: Outbreak of NDM-1-producing in a Dutch Hospital, with interspecies transfer of the resistance plasmid and unexpected occurrence in unrelated health care centers
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.00535-17
  contributor:
    fullname: Bosch
– volume: 30
  start-page: 3399
  year: 2014
  end-page: 3401
  ident: CR38
  article-title: Poretools: a toolkit for analyzing nanopore sequence data
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu555
  contributor:
    fullname: Quinlan
– year: 2019
  ident: CR24
  article-title: Hypervirulent
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.0001-19
  contributor:
    fullname: Marr
– volume: 58
  start-page: 6550
  year: 2014
  end-page: 6557
  ident: CR31
  article-title: Characterization of a P1-like bacteriophage carrying an SHV-2 extended-spectrum β-lactamase from an strain
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.03183-14
  contributor:
    fullname: Billard-Pomares
– year: 2020
  ident: CR21
  article-title: Molecular characteristics of carbapenemase-producing in the Netherlands; results of the 2014–2018 national laboratory surveillance
  publication-title: Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis.
  doi: 10.1016/j.cmi.2020.01.027
  contributor:
    fullname: van der Zwaluw
– volume: 45
  start-page: 131
  year: 2018
  end-page: 139
  ident: CR5
  article-title: as a key trafficker of drug resistance genes from environmental to clinically important bacteria
  publication-title: Curr. Opin. Microbiol.
  doi: 10.1016/j.mib.2018.04.004
  contributor:
    fullname: Holt
– volume: 30
  start-page: 2068
  year: 2014
  end-page: 2069
  ident: CR40
  article-title: Prokka: rapid prokaryotic genome annotation
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu153
  contributor:
    fullname: Seemann
– volume: 17
  start-page: 90
  year: 2019
  end-page: 93
  ident: CR26
  article-title: Coexistence of three KPC-2 genes on an IncF/IncR plasmid in ST11
  publication-title: J. Glob. Antimicrob. Resist.
  doi: 10.1016/j.jgar.2018.11.017
  contributor:
    fullname: Zong
– volume: 10
  start-page: 542
  year: 2019
  ident: CR43
  article-title: Diversity, virulence, and antimicrobial resistance in isolates from the newly emerging ST101 lineage
  publication-title: Front. Microbiol.
  doi: 10.3389/fmicb.2019.00542
  contributor:
    fullname: Roe
– volume: 14
  start-page: 265
  year: 2016
  end-page: 279
  ident: CR18
  article-title: Oxford nanopore MinION sequencing and genome assembly
  publication-title: Genom. Proteom. Bioinform.
  doi: 10.1016/j.gpb.2016.05.004
  contributor:
    fullname: Ning
– year: 2017
  ident: CR25
  article-title: Emergence and Evolution of Multidrug-Resistant with both KPC and CTX-M Integrated in the Chromosome
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.00076-17
  contributor:
    fullname: Huang
– volume: 9
  start-page: 18861
  year: 2019
  ident: CR32
  article-title: Diversity of P1 phage-like elements in multidrug resistant
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-019-54895-4
  contributor:
    fullname: Venturini
– year: 2017
  ident: CR27
  article-title: Mutations in KPC-3 that confer ceftazidime-avibactam resistance encode novel KPC-3 variants that function as extended-spectrum β-lactamases
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.02534-16
  contributor:
    fullname: Haidar
– volume: 60
  start-page: 470
  year: 2007
  end-page: 482
  ident: CR14
  article-title: Class A carbapenemases
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dkm226
  contributor:
    fullname: Høiby
– volume: 112
  start-page: E3574
  year: 2015
  end-page: 3581
  ident: CR23
  article-title: Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in , an urgent threat to public health
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1501049112
  contributor:
    fullname: Holt
– volume: 10
  start-page: e0123690
  year: 2015
  ident: CR33
  article-title: The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0123690
  contributor:
    fullname: van der Zwaluw
– volume: 237
  start-page: 470
  issue: 7
  year: 2017
  end-page: 475
  ident: CR44
  article-title: Clonal or not clonal? Investigating hospital outbreaks of KPC-producing with whole-genome sequencing
  publication-title: Clin. Microbiol. Infect.
  doi: 10.1016/j.cmi.2017.01.015
  contributor:
    fullname: Ruppé
– volume: 49
  start-page: 4760
  year: 2005
  end-page: 4762
  ident: CR15
  article-title: Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing beta-lactamase
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.49.11.4760-4762.2005
  contributor:
    fullname: Yamaguchi
– volume: 8
  start-page: 460
  year: 2017
  end-page: 469
  ident: CR1
  article-title: The global epidemiology of carbapenemase-producing
  publication-title: Virulence
  doi: 10.1080/21505594.2016.1222343
  contributor:
    fullname: Doi
– volume: 8
  start-page: 470
  year: 2017
  end-page: 484
  ident: CR4
  article-title: Therapeutic options for carbapenem-resistant infections
  publication-title: Virulence
  doi: 10.1080/21505594.2017.1292196
  contributor:
    fullname: Tumbarello
– volume: 13
  start-page: 785
  year: 2013
  end-page: 796
  ident: CR11
  article-title: Clinical epidemiology of the global expansion of carbapenemases
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(13)70190-7
  contributor:
    fullname: Munoz-Price
– ident: CR20
– volume: 67
  start-page: 2640
  year: 2012
  end-page: 2644
  ident: CR36
  article-title: Identification of acquired antimicrobial resistance genes
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dks261
  contributor:
    fullname: Zankari
– volume: 55
  start-page: 5370
  year: 2011
  ident: 73440_CR9
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.05202-11
  contributor:
    fullname: G Cuzon
– volume: 9
  start-page: 18861
  year: 2019
  ident: 73440_CR32
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-019-54895-4
  contributor:
    fullname: C Venturini
– volume: 8
  start-page: 460
  year: 2017
  ident: 73440_CR1
  publication-title: Virulence
  doi: 10.1080/21505594.2016.1222343
  contributor:
    fullname: D van Duin
– volume: 45
  start-page: 131
  year: 2018
  ident: 73440_CR5
  publication-title: Curr. Opin. Microbiol.
  doi: 10.1016/j.mib.2018.04.004
  contributor:
    fullname: KL Wyres
– ident: 73440_CR20
– volume: 24
  start-page: 944
  year: 2016
  ident: 73440_CR7
  publication-title: Trends Microbiol.
  doi: 10.1016/j.tim.2016.09.007
  contributor:
    fullname: KL Wyres
– volume: 22
  start-page: 123
  year: 2016
  ident: 73440_CR28
  publication-title: Microb. Drug Resist.
  doi: 10.1089/mdr.2015.0083
  contributor:
    fullname: E Kocsis
– volume: 66
  start-page: 1290
  year: 2018
  ident: 73440_CR3
  publication-title: Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am.
  doi: 10.1093/cid/cix893
  contributor:
    fullname: RA Bonomo
– volume: 276
  start-page: 269
  issue: Pt 1
  year: 1991
  ident: 73440_CR12
  publication-title: Biochem. J.
  doi: 10.1042/bj2760269
  contributor:
    fullname: RP Ambler
– volume: 6
  start-page: 1083
  year: 2017
  ident: 73440_CR19
  publication-title: F1000Research
  contributor:
    fullname: C de Lannoy
– volume: 55
  start-page: 2380
  year: 2017
  ident: 73440_CR16
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.00535-17
  contributor:
    fullname: T Bosch
– volume: 14
  start-page: 265
  year: 2016
  ident: 73440_CR18
  publication-title: Genom. Proteom. Bioinform.
  doi: 10.1016/j.gpb.2016.05.004
  contributor:
    fullname: H Lu
– volume: 43
  start-page: 4178
  year: 2005
  ident: 73440_CR34
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.43.8.4178-4182.2005
  contributor:
    fullname: L Diancourt
– volume: 65
  start-page: 387
  year: 2018
  ident: 73440_CR30
  publication-title: Acta Microbiol. Immunol. Hung.
  doi: 10.1556/030.65.2018.031
  contributor:
    fullname: A Hanczvikkel
– volume: 58
  start-page: 3895
  year: 2014
  ident: 73440_CR35
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.02412-14
  contributor:
    fullname: A Carattoli
– volume: 20
  start-page: 356
  year: 2019
  ident: 73440_CR17
  publication-title: Nat. Rev. Genet.
  contributor:
    fullname: M Boolchandani
– volume: 17
  start-page: 90
  year: 2019
  ident: 73440_CR26
  publication-title: J. Glob. Antimicrob. Resist.
  doi: 10.1016/j.jgar.2018.11.017
  contributor:
    fullname: Y Feng
– volume: 15
  start-page: e1008114
  year: 2019
  ident: 73440_CR29
  publication-title: PLoS Genet.
  doi: 10.1371/journal.pgen.1008114
  contributor:
    fullname: KL Wyres
– volume: 13
  start-page: 785
  year: 2013
  ident: 73440_CR11
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(13)70190-7
  contributor:
    fullname: LS Munoz-Price
– year: 2019
  ident: 73440_CR2
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1111/nyas.14223
  contributor:
    fullname: K Kopotsa
– year: 2020
  ident: 73440_CR21
  publication-title: Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis.
  doi: 10.1016/j.cmi.2020.01.027
  contributor:
    fullname: K van der Zwaluw
– volume: 13
  start-page: e1005595
  year: 2017
  ident: 73440_CR39
  publication-title: PLoS Comput. Biol.
  doi: 10.1371/journal.pcbi.1005595
  contributor:
    fullname: RR Wick
– volume: 58
  start-page: 6550
  year: 2014
  ident: 73440_CR31
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.03183-14
  contributor:
    fullname: T Billard-Pomares
– volume: 13
  start-page: 751
  year: 2016
  ident: 73440_CR37
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3930
  contributor:
    fullname: M Loose
– volume: 237
  start-page: 470
  issue: 7
  year: 2017
  ident: 73440_CR44
  publication-title: Clin. Microbiol. Infect.
  doi: 10.1016/j.cmi.2017.01.015
  contributor:
    fullname: E Ruppé
– volume: 52
  start-page: 1257
  year: 2008
  ident: 73440_CR10
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.01451-07
  contributor:
    fullname: T Naas
– volume: 112
  start-page: E3574
  year: 2015
  ident: 73440_CR23
  publication-title: Proc. Natl. Acad. Sci. U. S. A.
  doi: 10.1073/pnas.1501049112
  contributor:
    fullname: KE Holt
– volume: 30
  start-page: 2068
  year: 2014
  ident: 73440_CR40
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu153
  contributor:
    fullname: T Seemann
– volume: 56
  start-page: 2143
  year: 2012
  ident: 73440_CR45
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.05308-11
  contributor:
    fullname: A García-Fernández
– volume: 67
  start-page: 2640
  year: 2012
  ident: 73440_CR36
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dks261
  contributor:
    fullname: E Zankari
– volume: 73
  start-page: 1121
  year: 2018
  ident: 73440_CR8
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dkx488
  contributor:
    fullname: M Rozwandowicz
– volume: 70
  start-page: 84
  year: 2019
  ident: 73440_CR22
  publication-title: Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis.
  contributor:
    fullname: W Liang
– volume: 10
  start-page: 542
  year: 2019
  ident: 73440_CR43
  publication-title: Front. Microbiol.
  doi: 10.3389/fmicb.2019.00542
  contributor:
    fullname: CC Roe
– volume: 49
  start-page: 4760
  year: 2005
  ident: 73440_CR15
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.49.11.4760-4762.2005
  contributor:
    fullname: J Alba
– volume: 8
  start-page: 470
  year: 2017
  ident: 73440_CR4
  publication-title: Virulence
  doi: 10.1080/21505594.2017.1292196
  contributor:
    fullname: EM Trecarichi
– volume: 8
  start-page: e00489-17
  issue: 3
  year: 2017
  ident: 73440_CR42
  publication-title: mBio
  doi: 10.1128/mBio.00489-17
  contributor:
    fullname: SW Wesley Long
– volume: 44
  start-page: W16
  year: 2016
  ident: 73440_CR41
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkw387
  contributor:
    fullname: D Arndt
– year: 2019
  ident: 73440_CR24
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.0001-19
  contributor:
    fullname: TA Russo
– volume: 41
  start-page: 252
  year: 2017
  ident: 73440_CR6
  publication-title: FEMS Microbiol. Rev.
  doi: 10.1093/femsre/fux013
  contributor:
    fullname: S Navon-Venezia
– volume: 10
  start-page: e0123690
  year: 2015
  ident: 73440_CR33
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0123690
  contributor:
    fullname: K van der Zwaluw
– volume: 60
  start-page: 470
  year: 2007
  ident: 73440_CR14
  publication-title: J. Antimicrob. Chemother.
  doi: 10.1093/jac/dkm226
  contributor:
    fullname: J Walther-Rasmussen
– year: 2017
  ident: 73440_CR27
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.02534-16
  contributor:
    fullname: G Haidar
– volume: 30
  start-page: 3399
  year: 2014
  ident: 73440_CR38
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu555
  contributor:
    fullname: NJ Loman
– year: 2017
  ident: 73440_CR25
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.00076-17
  contributor:
    fullname: W Huang
– volume: 28
  start-page: 565
  year: 2015
  ident: 73440_CR13
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.00116-14
  contributor:
    fullname: AJ Mathers
SSID ssj0000529419
Score 2.4757717
Snippet Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention it is...
Abstract Carbapenemase-producing Klebsiella pneumoniae emerged as a nosocomial pathogen causing morbidity and mortality in patients. For infection prevention...
SourceID pubmedcentral
proquest
crossref
springer
SourceType Open Access Repository
Aggregation Database
Publisher
StartPage 16778
SubjectTerms 631/114
631/326
Humanities and Social Sciences
multidisciplinary
Science
Science (multidisciplinary)
SummonAdditionalLinks – databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1bSxwxGA2iFPoi2lpcLyVC3-q02SQzSR5EZK0sFYsPCr6FZPKJA-uoOzvi_oj-536ZS2VFxLcZZjKEnJPkZL4bId_SofaaZXkSpEsTacAnJkiWGC1zkwXFhhDjnc_-ZONL-fsqvVoifbmjbgCrV492sZ7U5XTy4-lhfogT_qANGdc_K9yEYqAYHoSUiLZKXJJXuBQyMv6sk_ttrm9u5NB0sTOvN13cn55F50uXyRd202Y7Olkjq52OpEct8OtkCcpP5ENbWXL-mfw9R1V8WwQaer8L2pQVosiXNmxxMqdNHAsEejoBXxXRDYrel1Dje4UD6ifu9HyUcOrK0N8IWiBXozylkUC4WGLzoqSoIulxjQSg_c9FWtXTR4gljZBVG-Ti5NfFaJx0lReSXBg2SySkTsrUCJzuwfDUmVRD7pVxOeN5yDI8jzN3zTLmlDLeZ0aYjCPaCheEoMUXslzelbBJqM5zXDcc-GuQ0nuj8YLJ6KgF4Bh3A_K9H2573-bXsI1dXGjbgmMRHNuAY_mA7PWIWJwG0bbhSrirK4sqJWZOk0oMiFqA6v9nYyLtxSdlcdMk1FapRJliBmS_B9X2RHyjM1vv6Mw2-cgjuRqnwR2yPJvWsIsqZua_NtT8B7u08Z4
  priority: 102
  providerName: Scholars Portal
– databaseName: Springer_OA刊
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1bSxwxGA1lpeCL1LbiWpUUfGsHs5P7o6yKVCr7YMG3kEy-xYF1FGdH8Ef4n_0yly0rUujbDJMMw5yT5CTfjZAjOTHBMFVkUXiZCQshs1GwzBpRWBU1m0CKd_59pS7-iF838qZPk5NiYdbs99wc17jApCAw3ORonuyQON1uyIliicFTNV2dpySLlZjYPi7m_a7ra89fQfnWHfKNTbRdas4_ka1eI9KTDtRt8gGqz-RjVzXy-Qt5maHivSsjjYNPBW1LBlHkQheSuHimbYwKRHq5gFCXycWJPlTQYLvSAw0LfzmbZjn1VRxuOC2Rh0l60kQOnAixe1lRVIj0tEFw6XBwSOvm8QlSuSJkzFdyfX52Pb3I-qoKWcEtW2YCpBdCWo5DOdpceisNFEFbX7C8iErhXpv5OVPMa21DUJZblSOSGgd7NHyHjKr7CnYJNUWBc4KHMAchQrAGL5hITlgAnuV-TH4Mv9s9dLkzXGvz5sZ14DgEx7XguHxMvg-IOKR4slv4Cu6b2qECSVnRhOZjotegWr02Jclef1KVt22ybC0FShA7Jj8HUF0_Sut_fMze_zX_RjbzxLPWN3CfjJaPDRygWFmGw5alr5wc5TM
  priority: 102
  providerName: Springer Nature
Title Plasmid diversity among genetically related Klebsiella pneumoniae blaKPC-2 and blaKPC-3 isolates collected in the Dutch national surveillance
URI https://link.springer.com/article/10.1038/s41598-020-73440-2
https://search.proquest.com/docview/2449959473
https://pubmed.ncbi.nlm.nih.gov/PMC7546619
Volume 10
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Ja9wwFBZJSqGX0pVOl0GF3lpnNFos6di6CaFhwlBSmJvQFmqYcYZ4XMiP6H_uk2y3nRx66MVYeBP6Pkvv6W0IvRNz5RQpfRG4FQXX0RU6cFJoxb0ugyTzmOKdFxfl2Tf-ZSVWB0iMsTDZad-7-rhZb46b-nv2rdxu_Gz0E5stF5UUHJYVPTtEh5Kxv1T0PqE31XyuhwAZwtSshUUqBZKBoiRZsmWmEjagxzNGNdtfj_4ImXddJO_YSfPyc_oIPRzkRvyx799jdBCbJ-h-X0ny9in6uQQpeFMHHEY_C5zLCGHgRx-muL7FOW4lBny-jq6tk9sT3jaxg_tqG7Fb2_NlVVBsmzA2GK6Bm0kcxYkwMDnC43WDQWrEnzsAHI-bibjtbn7EVMIIWPQMXZ6eXFZnxVBpofBMk13Bo7CcCw0jQoOmwmqhondSW0-oD2UJ-jexV6QkVkrtXKmZLimgK2ECCIo9R0fNdRNfIKy8h3nCRncVOXdOKzghPDlmxWgJtRP0fhxus-3zaZhsB2fK9DgZwMlknAydoLcjIgZon2wZtonXXWtAKkmZ0rhkEyT3oPr92pQ4e_8K8Ckn0B74M0EfRlDN8Oe2_-jMy__-0Cv0gCb2ZdfB1-hod9PFNyDL7NwUGLySU3Tv08nF8iu0qrKa5n0BOC64mmZu_wKbUvp-
link.rule.ids 230,315,733,786,790,870,891,24346,27955,27956,31753,33778,41153,42222,51609,53825,53827
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Jj9MwFLaGQQgu7IiyGokbpHW9JPERFUaFTkc9FDQ3y9toIlpPNWmQhv_Af-bZSYD2gAS3RNmcvM9vib_3HkKvxbg0Jclt5rgWGZfeZNJxksmSW5m7gox9zHeen-TTz_zTqTg9QKLPhUmkfWuqYVith6E6T9zKzdqOep7YaDGfFIKDWZGja-g6zFcq_gjS25LeVPKx7FJkCCtHNZipmEoGoVLB4mpmbGIDkTxjVLJdi_TbzdwnSe6tlCYDdHQHfemH3vJOvg6brRna73tVHf_53e6i251Lit-1h--hAx_uoxttk8qrB-jHAhzsdeWw6ykcOHUowgC9NgNydYVTSox3eLbypq4iowpvgm_gvEp7bFZ6tphkFOvg-h2GK4B99HRxxCLoXbi8ChgcUvy-ASzh_j8lrpvLbz52RwKAPkTLow_LyTTrmjhklkmyzbgXmnMh4VNTJ6nQUpTemkJqS6h1eQ6hPdFnJCe6KKQxuWQypwCcAnSLK9kjdBgugn-McGktqCDtzZnn3BhZwgbhkfPlvSZUD9CbXo5q05bqUGmJnZWqBYACAKgEAEUH6FUvagUzKi6T6OAvmlqBwxOLsPGCDVCxg4Fft401uXePgDhTbe5OfAP0tkeL6pRC_ZfBPPnvB71EN6fL-bE6_ngye4pu0QjxxFB8hg63l41_Di7T1rxIE-QnSyEXXA
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELagCMSF8hRbChiJG2TjtZ04PqItq8LSag9FqrhYfq0asZuumk2l8h_6nzt2EujugUNvifJyMp_HM5lvZhD6mI0KU5DcJo7rLOHSm0Q6ThJZcCtzJ8jIh3zno-P88Cf_fpqd3mr1FUn71pTDarEcVuVZ5FauljbteWLp7GgsMg7LikxXbp7eRw9gzlJxy1Fvy3pTyUeyS5MhrEhrWKpCOhm4S4KFiGZoZAPePGNUss1V6Z-puU2U3IqWxkVosot-9cNvuSe_h83aDO2frcqOd3q_p-hJZ5riL-0pz9A9Xz1HD9tmlVcv0PUMDO1l6bDrqRw4dirCAME2E3JxhWNqjHd4uvCmLgOzCq8q38B5pfbYLPR0Nk4o1pXrdxguAf7B4sUBk6B_4fKywmCY4oMGMIX7_5W4bi4ufeiSBEB9iU4mX0_Gh0nXzCGxTJJ1wn2mOc8kfG7qJM20zApvjZDaEmpdnoOLT_Sc5EQLIY3JJZM5BQAJ0DGuYK_QTnVe-dcIF9aCKtLezD3nxsgCNggP3C_vNaF6gD71slSrtmSHiqF2VqgWBApAoCIIFB2gD724FcysEC7RlT9vagWGTyjGxgUbILGBg7-3DbW5N4-ASGON7k6EA_S5R4zqlEP9n8Hs3flB79Gj2cFE_fh2PH2DHtOA8khU3Ec764vGvwXLaW3exTlyAwEkGdw
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Plasmid+diversity+among+genetically+related+Klebsiella+pneumoniae+blaKPC-2+and+blaKPC-3+isolates+collected+in+the+Dutch+national+surveillance&rft.jtitle=Scientific+reports&rft.au=Hendrickx%2C+Antoni+P+A&rft.au=Landman%2C+Fabian&rft.au=de+Haan%2C+Angela&rft.au=Borst%2C+Dyogo&rft.date=2020-10-08&rft.eissn=2045-2322&rft.volume=10&rft.issue=1&rft.spage=16778&rft.epage=16778&rft_id=info:doi/10.1038%2Fs41598-020-73440-2&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon