Inhibitory effect of luteolin on TNF-α-induced IL-8 production in human colon epithelial cells

• Published in: International immunopharmacology Vol. 5; no. 1; pp. 209 - 217
• Main Authors: Kim, Jin-A, Kim, Dae-Ki, Kang, Ok-Hwa, Choi, Yeon-A, Park, Hye-Jung, Choi, Suck-Chei, Kim, Tae-Hyun, Yun, Ki-Jung, Nah, Yong-Ho, Lee, Young-Mi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2005; Elsevier
• Subjects: Anti-Inflammatory Agents - pharmacology; Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; Cell Survival - drug effects; DNA - metabolism; HT29 Cells; Humans; I-kappa B Proteins - metabolism; Interleukin-8 (IL-8); Interleukin-8 - antagonists & inhibitors; Interleukin-8 - genetics; Interleukin-8 - metabolism; Intestinal epithelial cells; IκB; Luteolin; Luteolin - pharmacology; MAPK; Medical sciences; Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - antagonists & inhibitors; Mitogen-Activated Protein Kinase 3 - metabolism; NF-kappa B - metabolism; NF-κB; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Pharmacology. Drug treatments; Phosphorylation - drug effects; Protein Binding; RNA, Messenger - metabolism; Tumor Necrosis Factor-alpha; Interleukin-8 (IL-8); IL; Intestinal epithelial cells; IBD; MAPK; TNF; IECs; LPS; IκB; NF-κB; EMSA; Luteolin; ELISA; ERK; Human; Angiogenic factor; Digestive system; Enzyme; Cytokine; Gut; Mitogen-activated protein kinase; In vitro; Production; Epithelial cell; Colon; Transcription factor NFκB; Tumor necrosis factor α; Interleukin 8
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2004.09.027

Interleukin (IL)-8 plays a central role in the initiation and maintenance of inflammatory responses in the inflammatory bowel disease. The proinflammatory cytokine-mediated production of IL-8 requires activation of various kinases, which leads to the IκB degradation and NF-κB activation. In this study, we investigated the role of luteolin, a major flavonoid of Lonicera japonica, on TNF-α-induced IL-8 production in human colonic epithelial cells. HT29 cells were stimulated with TNF-α in the presence or absence of luteolin. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase–polymerase chain reaction (RT–PCR) analysis, and the mitogen-activated protein kinases (MAPKs) activation and IκB degradation were determined by Western blot analysis. NF-κB activation was assessed by the electrophoretic motility shift assay (EMSA). Luteolin suppressed TNF-α-induced IL-8 production in dose-dependent manner. In addition, luteolin inhibited TNF-α-induced phosphorylation of p38 MAPK and extracellular-regulated kinases (ERK), IκB degradation, and NF-κB activation. These results suggest that luteolin has the inhibitory effects on TNF-α-induced IL-8 production in the intestinal epithelial cells through blockade in the phosphorylation of MAPKs, following IκB degradation and NF-κB activation.