Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp

• Published in: Biotechnology letters Vol. 23; no. 20; pp. 1685 - 1689
• Main Authors: TECHAPUN, Charin, SINSUWONGWAT, Suphawat, POOSARAN, Naiyatat, WATANABE, Masanori, SASAKI, Ken
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.10.2001; Springer Nature B.V
• Subjects: Agricultural wastes; Bacteria; Bagasse; Biological and medical sciences; Biotechnology; Carbon sources; carboxymethylcellulose; Cellulose; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Microbiology; Microorganisms; Production of selected enzymes; Streptomyces; Waste materials; Thailand; Streptomycetaceae; Enzyme; Streptomyces; Endo-1,4-β-xylanase; Fermentation; Actinomycetales; Glycosidases; Production; Bacteria; Hydrolases; Actinomycetes; Agricultural waste; Thermotolerance; O-Glycosidases
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1023/A:1012448229555

1444 microorganisms were isolated from soil samples from the northern Thai and screened at 55 °C by using basal medium supplemented with 1% carboxymethyl cellulose as a sole carbon source. One isolate, Streptomyces Ab106, had a high activity of a cellulase-free xylanase also without mannanase activity. The maximum cellulase-free xylanase activities of 3.5, 3.3, 3.1 and 2.7 IU were after growth of the organism with 1% (w/v) corn hull, corncob, bagasse and oat spelt xylan, respectively, at 55 °C for 6 days, respectively. The activity was more than 5 times higher than that at 35 °C.[PUBLICATION ABSTRACT]