High-performance thin-layer chromatography method for monitoring norfloxacin residues on pharmaceutical equipment surfaces

• Published in: Journal of Chromatography A Vol. 862; no. 2; pp. 209 - 215
• Main Authors: Simonovska, Breda, Andrenšek, Samo, Vovk, Irena, Prošek, Mirko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 12.11.1999; Elsevier
• Subjects: Anti-Infective Agents - analysis; Antibiotics; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Densitometry; Drug Residues - analysis; Fluoroquinolones; General pharmacology; Image Processing, Computer-Assisted; Medical sciences; Norfloxacin; Norfloxacin - analysis; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Spectrometry, Fluorescence; Fluoroquinolones; Pharmaceutical analysis; Densitometry; Antibiotics; Norfloxacin; Trace analysis; Surface cleaning; Validation; Chemical analysis; Contamination; Thin layer chromatography; Fluoroquinolone derivatives; Ultraviolet detector; Analysis method; Residue; Stainless steel; Antibacterial agent; Videodensitometry; Quinolone derivatives; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(99)00935-8

This paper presents a high-performance thin-layer chromatography (HPTLC) method with direct fluorescence measurement for the determination of norfloxacin. The method was validated for the monitoring of norfloxacin residues on stainless steel surfaces at the allowed limit of 10 mg of norfloxacin per square meter. However, it can be adapted for lower amounts of residues owing to the low detection limit of norfloxacin (about 5 ng) and can also be used for other surface materials. Test solutions were analyzed by the new HPTLC method and the known HPLC method for comparison. Accuracy and precision of the new HPTLC method, with a subsequent quantification by densitometer or video system, are comparable with those of the HPLC method.