Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications

We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluoresc...

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Bibliographic Details
Published inOptics letters Vol. 39; no. 8; p. 2431
Main Authors Poland, Simon P, Krstajić, Nikola, Knight, Robert D, Henderson, Robert K, Ameer-Beg, Simon M
Format Journal Article
LanguageEnglish
Published United States 15.04.2014
Subjects
Algorithms
Animals
Image Processing, Computer-Assisted - statistics & numerical data
Larva - cytology
Microscopy, Fluorescence, Multiphoton - instrumentation
Microscopy, Fluorescence, Multiphoton - methods
Microscopy, Fluorescence, Multiphoton - statistics & numerical data
Muscle Fibers, Skeletal - cytology
Optical Phenomena
Zebrafish - anatomy & histology
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Summary:We report on the development of a doubly weighted Gerchberg-Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae.
ISSN:1539-4794
DOI:10.1364/ol.39.002431