Persistent infection of American bison (Bison bison) with bovine viral diarrhea virus and bosavirus

• Published in: Veterinary microbiology Vol. 252; p. 108949
• Main Authors: Hause, Ben M., Pillatzki, Angela, Clement, Travis, Bragg, Tom, Ridpath, Julia, Chase, Christopher C.L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021; Elsevier BV
• Subjects: 5' Untranslated Regions; Bison; Bison bison; Bosavirus; Bovine viral diarrhea virus; Buffalo; Cattle; Diarrhea; Genomes; Infections; Metagenomics; Parvovirus; Persistent infection; Reproductive failure; Respiratory diseases; Viruses; Oregon; United States > US; Bison; Bosavirus; Bovine viral diarrhea virus; Parvovirus; Persistent infection
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2020.108949

•Unthrifty bison were persistently infected with bovine viral diarrhea virus (BVDV).•The noncytopathic BVDV was 92.7 % similar to the vaccine strain Oregon C24V.•Bison were also persistently infected with bosavirus, a bovine parvovirus.•Persistent infection of bison with BVDV impacts eradication and control programs. Bovine viral diarrhea viruses (BVDV) are significant pathogens of cattle, leading to losses associated with reproductive failure, respiratory disease and immune dysregulation. While cattle are the reservoir for BVDV, a wide range of domestic and wild ruminants are susceptible to infection and disease caused by BVDV. Samples from four American bison (Bison bison) from a captive herd were submitted for diagnostic testing due to their general unthriftiness. Metagenomic sequencing on pooled nasal swabs and serum identified co-infection with a BVDV and a bovine bosavirus. The BVDV genome was more similar to the vaccine strain Oregon C24 V than to other BVDV sequences in GenBank, with 92.7 % nucleotide identity in the open reading frame. The conserved 5′-untranslated region was 96.3 % identical to Oregon C24 V. Bosavirus has been previously identified in pooled fetal bovine serum but its clinical significance is unknown. Sequencing results were confirmed by virus isolation and PCR detection of both viruses in serum and nasal swab samples from two of the four bison. One animal was co-infected with both BVDV and bosavirus while separate individuals were positive solely for BVDV or bosavirus. Serum and nasal swabs from these same animals collected 51 days later remained positive for BVDV and bosavirus. These results suggest that both viruses can persistently infect bison. While the etiological significance of bosavirus infection is unknown, the ability of BVDV to persistently infect bison has implications for BVDV control and eradication programs. Possible synergy between BVDV and bosavirus persistent infection warrants further study.