Onosma bracteatum Wall Aqueous-Ethanolic Extract Suppresses Complete Freund's Adjuvant-Induced Arthritis in Rats via Regulation of TNF-α, IL-6, and C-Reactive Protein

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 8; p. 1830
• Main Authors: Zafar, Farah, Shaheen, Ghazala, Asif, Hafiz Muhammad, Farhan, Mohd, Muteeb, Ghazala, Aatif, Mohammad
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.04.2024
• Subjects: Anemia; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants - chemistry; Antioxidants - pharmacology; arthritis; Arthritis, Experimental - chemically induced; Arthritis, Experimental - drug therapy; Arthritis, Experimental - pathology; C-Reactive Protein - metabolism; CFA; Cytokines; Drug withdrawal; Enzymes; Flavonoids; Freund's Adjuvant; Illnesses; Immune system; Inflammation; inflammatory mediators; Interleukin-6 - metabolism; Male; Nonsteroidal anti-inflammatory drugs; Onosma bracteatum wall; Oxidative stress; Phytochemicals; Phytochemicals - chemistry; Phytochemicals - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Proteins; qRT-PCR; Rats; Rats, Sprague-Dawley; Rheumatoid arthritis; Sapindaceae - chemistry; Sodium; Tumor Necrosis Factor-alpha - metabolism; Pakistan; India; inflammatory mediators; Onosma bracteatum wall; arthritis; qRT-PCR; CFA
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29081830

Wall ( ) has been used traditionally for the management of arthritis; however, its therapeutic potential warrants further investigation. This study aimed to evaluate the anti-arthritic effects of the aqueous-ethanolic extract of leaves (AeOB) in a rat model of complete Freund's adjuvant (CFA)-induced arthritis. Rats were treated with AeOB (250, 500, and 750 mg/kg), indomethacin (10 mg/kg), or a vehicle control from days 8 to 28 post-CFA injection. Arthritic score, paw diameter, and body weight were monitored at regular intervals. X-ray radiographs and histopathological analysis were performed to assess arthritic severity. Inflammatory cytokines tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP) were quantified by qPCR and icromatography. Phytochemical analysis of AeOB revealed alkaloids, flavonoids, phenols, tannins, Saponins, and glycosides. AeOB also exhibited antioxidant potential with an IC of 73.22 µg/mL in a DPPH assay. AeOB and diclofenac exhibited anti-inflammatory and anti-arthritic activities. Rats treated with AeOB at 750 mg/kg and indomethacin showed significantly reduced arthritic symptoms and joint inflammation versus the CFA control. The AeOB treatment downregulated TNF-α and IL-6 and decreased CRP levels compared with arthritic rats. Radiography and histopathology also showed improved prognosis. These findings demonstrate the anti-arthritic potential of AeOB leaves.