Co-expression and preferential interaction between two calcineurin B-like proteins and a CBL-interacting protein kinase from cotton

The CBL/CIPK signaling system mediates a variety of responses to environmental stimuli in plants. In this work, we identified four CBL genes from Gossypium hirsutum, two of which (designated GhCBL2 and GhCBL3) showed preferential expression in the elongating fiber cells. Moreover, the expression pat...

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Published inPlant physiology and biochemistry Vol. 46; no. 10; pp. 935 - 940
Main Authors Gao, Peng, Zhao, Pi-Ming, Wang, Juan, Wang, Hai-Yun, Du, Xiong-Ming, Wang, Gui-Ling, Xia, Gui-Xian
Format Journal Article
LanguageEnglish
Published Paris Elsevier Masson SAS 01.10.2008
Elsevier
Subjects
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Calcineurin - chemistry
Calcineurin - metabolism
Calcineurin B-Like protein
CBL-interacting protein kinase
Cotton fiber
DNA Primers
Fundamental and applied biological sciences. Psychology
Gossypium - enzymology
Gossypium - metabolism
Molecular Sequence Data
Plant physiology and development
Plant Proteins - chemistry
Plant Proteins - metabolism
Protein Binding
Sequence Homology, Amino Acid
CBL-interacting protein kinase
Cotton fiber
Calcineurin B-Like protein
Malvaceae
Enzyme
Transferases
Gossypium
Interaction
Non-specific serine/threonine protein kinase
Fiber crop
Protein
Plant physiology
Dicotyledones
Angiospermae
Spermatophyta
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Summary:The CBL/CIPK signaling system mediates a variety of responses to environmental stimuli in plants. In this work, we identified four CBL genes from Gossypium hirsutum, two of which (designated GhCBL2 and GhCBL3) showed preferential expression in the elongating fiber cells. Moreover, the expression patterns of these two CBL genes coincided with that of a putative CBL-interacting protein kinase gene ( GhCIPK1) that we isolated in a previous study. Yeast two-hybrid assay indicated that among the four CBLs, GhCIPK1 interacted selectively with GhCBL2 and GhCBL3. The co-expression and interactions of these proteins suggest that they are components of the same signaling pathway. These findings strengthen our previous prediction that CBL/CIPK signaling plays a critical role in the regulation of cotton fiber elongation.
Bibliography:ObjectType-Article-1
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ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2008.05.001