An injectable click-crosslinked hyaluronic acid hydrogel modified with a BMP-2 mimetic peptide as a bone tissue engineering scaffold

• Published in: Acta biomaterialia Vol. 117; pp. 108 - 120
• Main Authors: Park, Seung Hun, Park, Joon Yeong, Ji, Yun Bae, Ju, Hyeon Jin, Min, Byoung Hyun, Kim, Moon Suk
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2020; Elsevier BV
• Subjects: Availability; Biocompatibility; Biomedical materials; BMP-2 mimetic peptide; Bone morphogenetic protein 2; Bone morphogenetic proteins; Bone tissue engineering; Bones; Click-crosslinking; Crosslinking; Dental pulp; Differentiation (biology); Fluorescence; Human dental pulp stem cells; Humans; Hyaluronic Acid; Hydrogels; Hydrogels - pharmacology; Injectability; Injectable hydrogel scaffold; Osteogenesis; Osteogenic differentiation; Peptides; Physical properties; Scaffolds; Stem cells; Tissue Engineering; Tissue Scaffolds; Injectable hydrogel scaffold; Human dental pulp stem cells; Bone tissue engineering; Click-crosslinking; BMP-2 mimetic peptide; Osteogenic differentiation
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2020.09.013

An injectable, click-crosslinking (Cx) hyaluronic acid (HA) hydrogel scaffold modified with a bone morphogenetic protein-2 (BMP-2) mimetic peptide (BP) was prepared for bone tissue engineering applications. The injectable click-crosslinking HA formulation was prepared from HA-tetrazine (HA-Tet) and HA-cyclooctene (HA-TCO). The Cx-HA hydrogel scaffold was prepared simply by mixing HA-Tet and HA-TCO. The Cx-HA hydrogel scaffold was stable for a longer period than HA both in vitro and in vivo, which was verified via in-vivo fluorescence imaging in real time. BP acted as an osteogenic differentiation factor for human dental pulp stem cells (hDPSCs). After its formation in vivo, the Cx-HA scaffold provided a fine environment for the hDPSCs, and the biocompatibility of the hydrogel scaffold with tissue was good. Like traditional BMP-2, BP induced the osteogenic differentiation of hDPSCs in vitro. The physical properties and injectability of the chemically loaded BP for the Cx-HA hydrogel (Cx-HA-BP) were nearly identical to those of the physically loaded BP hydrogels and the Cx-HA-BP formulation quickly formed a hydrogel scaffold in vivo. The chemically loaded hydrogel scaffold retained the BP for over a month. The Cx-HA-BP hydrogel was better at inducing the osteogenic differentiation of loaded hDPSCs, because it prolonged the availability of BP. In summary, we successfully developed an injectable, click-crosslinking Cx-HA hydrogel scaffold to prolong the availability of BP for efficient bone tissue engineering. [Display omitted]