Fluorescence property of oxazole yellow-linked oligonucleotide. Triple helix formation and photocleavage of double-stranded DNA in the presence of spermine

• Published in: Bioorganic & medicinal chemistry Vol. 7; no. 6; pp. 1207 - 1211
• Main Authors: Inoue, Teruhiko, Sugiura, Yukio, Saitoh, Juichi, Ishiguro, Takahiko, Otsuka, Masami
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.1999; Elsevier Science
• Subjects: Analytical, structural and metabolic biochemistry; Benzoxazoles - pharmacology; Biological and medical sciences; DNA - chemistry; DNA - drug effects; DNA photocleavafge; Dna, deoxyribonucleoproteins; fluorescence; Fundamental and applied biological sciences. Psychology; Intercalating Agents - pharmacology; Nucleic Acid Conformation; Nucleic acids; oligonucleotide; Oligonucleotides - chemistry; Oxazole yellow; Photochemistry; Quinolines - pharmacology; Spectrometry, Fluorescence; Spermine - chemistry; triple helix; triple helix; DNA photocleavafge; Oxazole yellow; fluorescence; oligonucleotide; Molecular hybridization; Polyamine; Intercalating agent; Organic dye; Transcription promoter; Triplet; Scission; Double stranded DNA; Spermine; Interleukin 2; Fluorescent labelling; Cleavage; Oligonucleotide; Oligodeoxyribonucleotide
• ISSN: 0968-0896; 1464-3391
• DOI: 10.1016/S0968-0896(99)00035-8

Oxazole yellow is an intercalator that shows enhanced fluorescence upon binding to DNA. We prepared an oxazole yellow-linked oligonucleotide that can form triple helix with interleukin 2 receptor α chain promoter. The oxazole yellow-linked oligonucleotide showed linear increase of fluorescence by the triple helix formation with double-stranded DNA and also induced photocleavage of the targeted DNA in the presence of spermine upon visible illumination. Cleavage site of one strand was 7 or 8 bases away from the site of intercalation whereas the other strand was cleaved at the intercalated site.