The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

• Published in: Veterinary microbiology Vol. 216; pp. 38 - 44
• Main Authors: Guerra, Priscila Regina, Herrero-Fresno, Ana, Pors, Susanne Elisabeth, Ahmed, Shahana, Wang, Dan, Thøfner, Ida, Antenucci, Fabio, Olsen, John Elmerdahl
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2018; Elsevier BV
• Subjects: Biosynthesis; Birds; Chicken; Chickens; Colonization; E coli; Embryo; Histology; Histopathology; Lesions; Liver; Lungs; Membrane proteins; Mutants; Organs; Pathogenesis; Polyamines; Poultry; Putrescine; Spermidine; Spleen; Studies; Virulence; Embryo; Chicken; Histopathology; Putrescine; Spermidine; E. coli
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2018.01.011

•A polyamine transport mutant, ΔpotE, showed reduced colonization and less pathology in the lung of challenged chicken.•A polyamine biosynthesis mutant, ΔspeB/C, demonstrated a decrease of colonization in the liver and spleen.•The Pot-membrane system is a potentially a good target for antimicrobials. Over the last few years, polyamines have been described as key-signal of virulence in pathogenic bacteria. In the current study, we investigated whether the knockout of genes related to polyamine biosynthesis and putrescine transport affected the virulence of an avian pathogenic E. coli (APEC) strain. One-week-old White Leghorn chickens were infected intratracheally with mutants in polyamine biosynthesis (ΔspeB/C and ΔspeD/E) and transport genes (ΔpotE) of a well-characterized APEC strain of ST117 (O83: H4). All polyamine mutants and the wild-type strain were able to infect chicken; however, we observed significantly fewer lesions in the lungs of the chickens infected with the polyamine mutants in comparison with chicken infected with the wild-type. Results derived from histology of infected lungs detected significantly fewer lesions in the lung of birds infected within particular the putrescine transport mutant (ΔpotE). A decrease in colonization levels was observed in the liver and spleen of birds infected with the putrescine biosynthesis mutant ΔspeB/C, and likewise, a decrease of the colonization levels of all organs from birds infected with the ΔpotE was detected. Together, our data demonstrate that the deletion of polyamine genes, and in particular the PotE membrane protein, attenuates the virulence of APEC during infection of chickens.