Platelet Membrane Early Activation Markers during Prolonged Storage

• Published in: Thrombosis research Vol. 93; no. 4; pp. 151 - 160
• Main Authors: Matsubayashi, Hidehiko, Weidner, John, Miraglia, Charles C., McIntyre, John A.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Ltd 15.02.1999; Elsevier Science
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Annexin A5; Annexin V; Antigens, CD; Biological and medical sciences; Biomarkers; Blood Platelets - metabolism; Blood Preservation; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; CD62P; CD63; Cell Membrane - metabolism; Cellular Senescence; Flow Cytometry; Humans; Medical sciences; P-Selectin; Phosphatidylserine; Platelet Activation; Platelet Membrane Glycoproteins; Tetraspanin 30; Thrombin; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; CD63; Thrombin; ATP, adenosine 5′-triphosphate; TB, Tyrode’s buffer; PS, phosphatidylserine; PE, phycoerythrin; APs, apheresis platelets; PCs, platelet concentrates; FITC, fluorescein-isothiocyanate; Annexin V; GPRP, Glycyl-L-prolyl-L-arginyl-L-proline; CD62P; Phosphatidylserine; GP, glycoprotein; Blood product; Human; Flow cytometry; Transfusion; Serine endopeptidases; Enzyme; Annexine V; Biological activity; Peptidases; Blood cell; Platelet; Investigation method; Plasma membrane; Hydrolases; Sample conservation
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/S0049-3848(98)00173-X

The relationship between platelet aging and early markers of membrane activation have not been defined clearly. Activation markers expressed during prolonged storage are similar if not identical to those that appear after exposure to thrombin. Using flow cytometry, we investigated platelet membrane expression of CD62P, CD63, and annexin V binding (i.e., loss of membrane asymmetry) in platelets stored for up to 11 days under standard blood banking conditions. We compared five apheresis platelets to two random donor platelet concentrates, and to one pooled platelet preparation from six single platelet concentrates before and after exposure to thrombin. CD62P, CD63 expression, and annexin V binding increased during storage albeit with different kinetics. The differential increments observed between resting and thrombin (1 unit/ml) activated platelets showed an inverse correlation to storage time for CD62P, CD63, and annexin V binding, which was identical to published survival curves. A difference between apheresis platelets and platelet concentrates was observed only on day 1. Our data indicate that the in vitro platelet reserve activity to thrombin activation mirrors that of radiolabeled platelet survival in vivo and that platelet cross-sectional residual life span can explain their diminished capacity to respond to thrombin as a function of viability.