A rapid high performance liquid chromatographic (HPLC) assay for the determination of oxytetracycline in commercial pharmaceuticals

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 23; no. 2; pp. 275 - 280
• Main Authors: Papadoyannis, I.N, Samanidou, V.F, Kovatsi, L.A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.2000; Elsevier Science
• Subjects: Analysis; Anti-Bacterial Agents - analysis; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Calibration; Chromatography, High Pressure Liquid - methods; General pharmacology; High performance liquid chromatography (HPLC); Medical sciences; Oxytetracycline; Oxytetracycline - analysis; Pharmaceutical Preparations - chemistry; Pharmaceuticals; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Oxytetracycline; High performance liquid chromatography (HPLC); Pharmaceuticals; Validation; Internal standard; Tetracycline derivatives; HPLC chromatography; Capsule; Ointment; Powder; Antibiotic; Reversed phase chromatography; Ultraviolet detector; Dosage form; Tablet; Antibacterial agent; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(00)00300-9

In the present study, a simple, sensitive and rapid reversed-phase high performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of oxytetracycline (OTC) is developed and applied to the determination of the antibiotic in commercial pharmaceutical preparations (powder, capsules, vaginal tablets and ointment). The isocratic elution is performed with methanol-0.01 M oxalic acid, pH 3.0 (30:70, v/v) at a flow rate of 0.95 ml/min, using a Silasorb C 8 analytical column, 250×4 mm, 10 μm. Codeine is used as internal standard. Absorbance is monitored at 250 nm where both analyte of interest and internal standard have significant absorption. Total analysis time was ∼7 min. Data with respect to precision and accuracy and limits of detection are reported and discussed. The described method can be readily utilised for analysis of pharmaceutical products and pharmacokinetic studies as well.