Quantitative determination of Closantel residues in milk by high-performance liquid chromatography with fluorescence detection

• Published in: Journal of Chromatography A Vol. 846; no. 1; pp. 383 - 386
• Main Authors: Stoev, G, Dakova, T, Michailova, Al
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 18.06.1999; Elsevier
• Subjects: Animals; Anthelmintics - analysis; Anthelmintics - pharmacokinetics; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Closantel; Food industries; Fundamental and applied biological sciences. Psychology; Milk - chemistry; Milk and cheese industries. Ice creams; Reproducibility of Results; Salicylanilides - analysis; Salicylanilides - pharmacokinetics; Sensitivity and Specificity; Sheep; Spectrometry, Fluorescence; Closantel; Extraction methods; Food analysis; Milk; Solid phase extraction; Fluorescence detector; Chemical analysis; Anthelmintic; Liquid liquid extraction; HPLC chromatography; Foodstuff; Solvent extraction; Veterinary medicine; Iodine Organic compounds; Contamination; Chemical enrichment; Analysis method; Sample preparation; Residue; Parasiticid; Benzamide derivatives; Carboxamide; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(99)00135-1

A HPLC method with fluorescence detection for quantitative determination of Closantel residues in milk has been developed and validated. The proposed cleaning procedure with acetonitrile and acetone extraction, and solid-phase clean-up with Florisil enables concentrations of Closantel below 50 μg/l to be determined. The method was shown to be sufficient, precise, accurate, selective and rugged. The method was applied in the regular monitoring of Closantel residues in milk and of the pharmacokinetic behavior of Closantel in sheep.