Molecular, chemical, and anatomical characterization of globus pallidus dopamine D2 receptor mRNA-containing neurons

• Published in: Synapse (New York, N.Y.) Vol. 52; no. 2; pp. 100 - 113
• Main Authors: Hoover, Brian R., Marshall, John F.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2004
• Subjects: Animals; basal ganglia; Globus Pallidus - chemistry; Globus Pallidus - cytology; Globus Pallidus - metabolism; in situ hybridization; Male; Neurons - chemistry; Neurons - cytology; Neurons - metabolism; parvalbumin; preproenkephalin; Protein Binding - physiology; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D2 - analysis; Receptors, Dopamine D2 - metabolism; RNA, Messenger - analysis; RNA, Messenger - metabolism; striatum; subthalamic nucleus
• ISSN: 0887-4476; 1098-2396
• DOI: 10.1002/syn.20007

Essential for normal movement, the globus pallidus (GP) is a prominent nucleus whose neurons project to all other basal ganglia nuclei. The GP is composed of at least two distinct neuron populations. GP neurons of the rodent contain either the calcium‐binding protein parvalbumin (PV) or preproenkephalin (PPE) mRNA, differentially innervate several basal ganglia structures, and have distinct immediate early gene responses to dopamine agonists or antagonists. Recent research has revealed that dopamine directly influences GP neurons, with D2 receptors contributing to both pre‐ and postsynaptic effects of dopaminergic agents. The existence of D2 mRNA‐expressing (D2+) GP neurons has been established, but little is known concerning their numbers, regional distribution, or relationship to pallidal subpopulations identified on the basis of PV immunocytochemistry, PPE mRNA, or axonal targets. Detection of pallidal D2 mRNA with a 35S‐cRNA probe revealed that D2+ neurons are found throughout the GP, comprising approximately one‐half of pallidal neurons, but they are most dense within a dorsoventral band in lateral GP. While a substantial proportion (42–51%) of all chemically and anatomically labeled pallidal neuron subpopulations expressed D2 transcript, the D2+ neurons exhibited both population‐based and regional heterogeneities. Overall, the pallidostriatal cells had a greater density of D2 mRNA than did pallidosubthalamic cells. Also, compared to other pallidal regions, the ventromedial GP contained fewer D2+ cells, and the PPE mRNA‐expressing cells in this region had lower densities of D2 mRNA per neuron. These results reveal heterogeneous chemical and anatomical properties of the extensive population of D2+ GP neurons, a potential cellular substrate for dopamine's effects in pallidum. Synapse 52:100–113, 2004. © 2004 Wiley‐Liss, Inc.