Primary structure of two major glycans of bovine fibrinogen

After pronase digestion of bovine fibrinogen, the asparagine‐linked glycans were released from the resulting glycopeptides by hydrazinolysis, and subsequently re‐N‐acetylated. Two sialylated glycans were isolated by ion‐exchange chromatography. Their primary structure has been determined by methylat...

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Bibliographic Details
Published inEuropean journal of biochemistry Vol. 151; no. 3; pp. 607 - 611
Main Authors Debeire, P, Montreuil, J, Moczar, E, Van Helbeek, H, Vliegenthart, J.F.G
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 16.09.1985
Blackwell
Subjects
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Carbohydrate Sequence
Cattle
Chromatography, Ion Exchange
Chromatography, Thin Layer
Fibrinogen
Fundamental and applied biological sciences. Psychology
Glycopeptides - analysis
Glycoproteins
Hydrazines
Hydrolysis
N-Acetylneuraminic Acid
Oligosaccharides - analysis
Peptide Fragments - analysis
Polysaccharides - analysis
Proteins
Sialic Acids - analysis
Bovine
Molecular structure
Animal
Fibrinogen
Primary structure
Glycoproteins
NMR spectrometry
Methylation
Glycan
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Summary:After pronase digestion of bovine fibrinogen, the asparagine‐linked glycans were released from the resulting glycopeptides by hydrazinolysis, and subsequently re‐N‐acetylated. Two sialylated glycans were isolated by ion‐exchange chromatography. Their primary structure has been determined by methylation analysis and 360‐MHz 1H‐NMR spectroscopy. The structures proposed to be present in the native glycoprotein are as follows:
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1985.tb09147.x