GARDIMYCIN, A NEW ANTIBIOTIC FROM ACTINOPLANES II. ISOLATION AND PRELIMINARY CHARACTERIZATION

The strain Actinoplanes garbadinensis nov. sp. produces a peptide antibiotic, named gardimycin, which is active in vitro and in vivo against Gram-positive bacteria. Isolation and purification of the product have been accomplished by extraction from the broth with butanol and dialysis of the crude ex...

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Bibliographic Details
Published inJournal of antibiotics Vol. 29; no. 5; pp. 507 - 510
Main Authors CORONELLI, C., TAMONI, G., LANCINI, G. C.
Format Journal Article
LanguageEnglish
Published Japan JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1976
Subjects
Actinomycetales
Amino Acids - analysis
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - isolation & purification
Chemical Phenomena
Chemistry
Chromatography, Paper
Chromatography, Thin Layer
Crystallization
Culture Media - analysis
Hydrolysis
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Summary:The strain Actinoplanes garbadinensis nov. sp. produces a peptide antibiotic, named gardimycin, which is active in vitro and in vivo against Gram-positive bacteria. Isolation and purification of the product have been accomplished by extraction from the broth with butanol and dialysis of the crude extract, followed by counter-current distribution. Gardimycin is an open chain peptide with an approximate minimal formula C84 H138N18S3-4O34Na. The following amino acids have been identified by column chromatography of an acid hydrolysate: serine, glutamic acid, alanine, leucine, isoleucine, glycine, valine and two sulphur-containing amino acids whose structure is presently under study. Tryptophan has been identified in an alkaline hydrolysate.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.29.507