Accounting for co-extractable compounds (blank correction) in spectrophotometric measurement of extractable and total-bound proanthocyanidin in Leucaena spp

• Published in: Journal of the science of food and agriculture Vol. 82; no. 8; pp. 860 - 868
• Main Authors: Dalzell, Scott A, Kerven, Graham L
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.06.2002; Wiley
• Subjects: absorbance; anthocyanidins; Biological and medical sciences; blank correction; bound; butanol; co-extractable compounds; computer software; condensed tannin; extractable; fibre-bound; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; hydrochloric acid; insoluble; Leucaena; Leucaena magnifica; methodology; Methods of analysis, processing and quality control, regulation, standards; proanthocyanidin; protein-bound; temperature; Interference; Leucaena; Correction factor; Accuracy; Analysis method; Leguminosae; Dicotyledones; Angiospermae; Pigments; Anthocyanidin; Spermatophyta; Proanthocyanidin; Measurement method; Spectrophotometry

Methods to account for the spectral interference of co-extractable compounds (blank correction) in the spectrophotometric analysis of both extractable and bound proanthocyanidin (PA) using the proanthocyanidin (butanol/HCl) assay were evaluated. Crude extractable and bound PA sample matrices of PA-f...