Wistar Rats Hippocampal Neurons Response to Blood Low-Density Polyethylene Microplastics: A Pathway Analysis of SOD, CAT, MDA, 8-OHdG Expression in Hippocampal Neurons and Blood Serum Aβ42 Levels

• Published in: Neuropsychiatric disease and treatment Vol. 19; pp. 73 - 83
• Main Authors: Sincihu, Yudhiakuari, Lusno, Muhammad Farid Dimjadi, Mulyasari, Tri Marthy, Elias, Saliza Mohd, Sudiana, I Ketut, Kusumastuti, Kurnia, Sulistyorini, Lilis, Keman, Soedjajadi
• Format: Journal Article
• Language: English
• Published: New Zealand Taylor & Francis Ltd 2023; Dove; Dove Medical Press
• Subjects: Alzheimer's disease; amyloid beta 1-42; Animal cognition; Antibodies; Bisphenol A; Blood; Brain research; Catalase; Chloramphenicol O-acetyltransferase; Cognitive ability; Deoxyguanosine; environmental pollution; Enzymes; Experimental research; Free radicals; hippocampal neuronal damage; Hippocampus; Low density polyethylenes; Membranes; microplastics; Neurons; Original Research; Oxidative stress; Particle size; Pathogenesis; pathway analysis; PCB; Plastic pollution; Polychlorinated biphenyls; Polyethylene; Proteins; Superoxide dismutase; microplastics; hippocampal neuronal damage; pathway analysis; amyloid beta 1-42; environmental pollution
• ISSN: 1176-6328; 1178-2021; 1178-2021
• DOI: 10.2147/NDT.S396556

Low-density polyethylene microplastics are ingested into the bloodstream and distributed to all the organ tissue, including the hippocampus, causing toxic effects. This research aimed to elucidate the responses of hippocampal neurons to microplastic in the blood based on the expressions of superoxide dismutase (SOD), catalase (CAT) enzymes, malondialdehyde (MDA), 8-oxo-7,8-dihydro-2-deoxyguanosine (8-OHdG) in hippocampal neurons, and blood serum amyloid beta 1-42 (Aβ42) levels using SMART PLS pathway analysis. This was a pure experimental research on Wistar rats with a post-test control group design. Five experimental groups (X1, X2, X3, X4, X5) were given 0.0375 mg, 0.075 mg, 0.15 mg, 0.3 mg, and 0.6 mg of low-density polyethylene microplastics mixed in 2cc distilled water, respectively. Furthermore, except for control (C), the groups received microplastics an oral probe for 90 days. The molecular response of hippocampal neurons of Wistar rats to microplastics in the blood significantly decreased SOD enzyme expression, while CAT enzyme was unaffected. It considerably increased neuronal membrane damage (expression of MDA), increased considerably neuronal deoxyribonucleic acid damage (expression of 8-OHdG), and decreased blood serum Aβ42 levels (pathway analysis, all t-value >1.96). The pathway analysis showed that hippocampal neurons were significantly affected by microplastic particles in the blood.