Genome‐wide identification and coexpression network analysis of heat shock protein superfamily in Apolygus lucorum

• Published in: Archives of insect biochemistry and physiology Vol. 116; no. 4; pp. e22145 - n/a
• Main Authors: Li, Yuan‐Yuan, Deligeer, Liu, Jing, Shi, Kai
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.08.2024
• Subjects: Animals; Apolygus lucorum; Bias; coexpression network; Crop damage; Damage tolerance; Environmental management; Environmental stress; gene family; Gene Regulatory Networks; Genes; Genome, Insect; Genomes; Genomic analysis; genomics; Heat; heat shock protein; Heat shock proteins; heat stress; Heat-Shock Proteins - genetics; Heat-Shock Proteins - metabolism; Heteroptera - genetics; Heteroptera - metabolism; Host plants; Hsp60 protein; Hsp70 protein; Hsp90 protein; insect biochemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insecticide resistance; Insecticides; Insects; Multigene Family; Network analysis; Pest control; Phylogeny; stress response; superfamily; temperature; Temperature effects; Apolygus lucorum; coexpression network; heat shock protein; gene family
• ISSN: 0739-4462; 1520-6327; 1520-6327
• DOI: 10.1002/arch.22145

Heat shock proteins (Hsp) function as crucial molecular chaperones, playing pivotal roles in insects' response to stress stimuli. Apolygus lucorum, known for its broad spectrum of host plants and significant crop damage potential, presents a compelling subject for understanding stress response mechanisms. Hsp is important for A. lucorum to tolerate temperature and insecticide stress and may be involved in the formation of resistance to the interactive effects of temperature and insecticide. Here, we employed comprehensive genomic approaches to identify Hsp superfamily members in its genome. In total, we identified 42 Hsp genes, including 3 Hsp90, 16 Hsp70, 13 Hsp60, and 10 Hsp20. Notably, we conducted motif analysis and gene structures for Hsp members, which suggested the same families are relatively conserved. Furthermore, leveraging the weighted gene coexpression network analysis, we observed diverse expression patterns of different Hsp types across various tissues, with certain Hsp70 showing tissue‐specific bias. Noteworthy among the highly expressed Hsp genes was testis‐specific, which may serve as a pivotal hub gene regulating the gene network. Our findings shed light on the molecular evolutionary dynamics and temperature stress response mechanisms of Hsp genes in A. lucorum, offering insights into its adaptive strategies and potential targets for pest management. 1. The genome‐wide analysis of the heat shock proteins (Hsp) superfamily were performed in the four hemipteran species. 2. Hsp70 stood as male and female gonad expression bias in the Hsp members for Apolygus lucorum. 3. Our results revealed two hub genes, which may contribute to sperm formation and protection under environmental stress. Research Highlights Heat shock proteins (Hsp) play a crucial role in the regulatory network to face environmental stress. In this study, first, we performed the genome‐wide analysis of the Hsp superfamily in the four hemipteran species. Second, the phylogenetic analysis showed that Hsps within the same group shared similar gene structures and motifs in Apolygus lucorum. Importantly, Hsp70 stood as male and female gonad expression bias in the Hsp members. Finally, the weighted gene correlation network analysis (WGCNA) results revealed two hub genes, which may contribute to sperm formation and protection under environmental stress.