Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Tran...

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Published inBiotechnology progress Vol. 34; no. 5; pp. 1129 - 1136
Main Authors Su, Chia‐Hung, Nguyen, Hoang Chinh, Nguyen, My Linh, Tran, Phung Thanh, Wang, Fu‐Ming, Guan, Yu‐Lin
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.09.2018
Wiley Subscription Services, Inc
Subjects
Acid production
Biomedical materials
Cosmetics
Detergents
Enzymatic activity
Enzyme activity
Enzymes
fatty acid
Fatty acids
Fatty Acids - chemistry
Fatty Acids - metabolism
gac oil
Hydrolysis
Industrial applications
Lipase
Lipase - metabolism
liquid lipase
Mathematical models
Oil
oil hydrolysis
Oils & fats
Optimization
Organic chemistry
Reaction time
Response surface methodology
gac oil
fatty acid
oil hydrolysis
liquid lipase
response surface methodology
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Summary:Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Transform lipase, a new and low‐cost liquid lipase formulation, was used for the first time in oil hydrolysis with gac oil as a triglyceride source in this study. Response surface methodology was employed to optimize the reaction conditions and establish a reliable mathematical model for predicting hydrolysis yield. A maximal yield of 94.16% was obtained at a water‐to‐oil molar ratio of 12.79:1, reaction temperature of 38.9 °C, enzyme loading of 13.88%, and reaction time of 8.41 h. Under this optimal reaction condition, Eversa Transform lipase could be reused for up to eight cycles without significant loss in enzyme activity. This study indicates that the use of liquid Eversa Transform lipase in enzyme‐catalyzed oil hydrolysis could be a promising and cheap method of fatty acid production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018
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ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.2714