Development of microsatellite markers for Dimorphandra mollis (Leguminosae), a widespread tree from the Brazilian cerrado

• Premise of the study: Microsatellite markers were developed for Dimorphandra mollis (Leguminosae), a widespread tree in the Brazilian cerrado (a savanna‐like vegetation). • Methods and Results: Microsatellite markers were developed from an enriched library. The analyses of polymorphism were based...

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Published inAmerican journal of botany Vol. 99; no. 3; pp. e102 - e104
Main Authors Souza, Helena A. V., Collevatti, Rosane G., Lemos‐Filho, José P., Santos, Fabrício R., Lovato, Maria Bernadete
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.03.2012
Botanical Society of America, Inc
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Summary:• Premise of the study: Microsatellite markers were developed for Dimorphandra mollis (Leguminosae), a widespread tree in the Brazilian cerrado (a savanna‐like vegetation). • Methods and Results: Microsatellite markers were developed from an enriched library. The analyses of polymorphism were based on 56 individuals from three populations. Nine microsatellite loci were polymorphic, with the number of alleles per locus ranging from three to 10 across populations. The observed and expected heterozygosities per locus and population ranged from 0.062 to 0.850 and from 0.062 to 0.832, respectively. • Conclusions: These microsatellites provide an efficient tool for population genetics studies and will be used to assess the genetic diversity and spatial genetic structure of D. mollis.
Bibliography:http://www.amjbot.org/site/misc/ifora.xhtml#2OpenAccessPolicy
Freely available online through the AJB Open Access option
The authors thank FAPEMIG (APQ‐01741‐10), CNPq (472423/2010‐0), and PRONEX (História Natural grant CBB‐APQ‐00336‐09) for financial support. We would also like to thank A. V. Chaves, S. T. Vilaça, B. R. N. Chaves, C. C. Lara, E. M. Martins, and R. L. Brandão for technical support in the generation of the microsatellite‐enriched libraries and clone sequences.
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ISSN:0002-9122
1537-2197
1537-2197
DOI:10.3732/ajb.1100413