Sequential Change in Action Potential of Rabbit Epicardium During and Following Radiofrequency Ablation
Action Potential Change During RF Ablation. introduction: Although radiofrequency (RF) catheter ablation is used to treat certain cardiac arrhythmias, little is known regarding transient changes in cellular electrophysiology during and following RF delivery. Optical recordings of action potential (O...
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Published in | Journal of cardiovascular electrophysiology Vol. 10; no. 9; pp. 1252 - 1261 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.09.1999
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Subjects | |
Online Access | Get full text |
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Summary: | Action Potential Change During RF Ablation. introduction: Although radiofrequency (RF) catheter ablation is used to treat certain cardiac arrhythmias, little is known regarding transient changes in cellular electrophysiology during and following RF delivery. Optical recordings of action potential (OAP) with voltage‐sensitive dyes allow immunity from electrical noise during RF delivery. The purpose of this study was to clarify the possible synergistic effects of both the thermal and electrotonic components of RF ablation.
Methods and Results: In this study, OAPs were recorded on the epicardium of 16 isolated Langendorff‐perfused rabbit hearts within or adjacent to lesions made by RF catheters. Hearts were perfused at room temperature with Tyrode's solution containing 2,3‐butanedione monoxime and stained by the voltage‐sensitive dye di‐4‐ANEPPS. OAPs were recorded before, during, and after RF pulses. Within the lesion, the action potential duration at 80% repolarization (APD80) of OAP decreased rapidly during the RF pulse, without recovery following the pulse. In the border zone surrounding the lesion, the RF energy resulted in a rapid decrease in APD80, which recovered promptly after the pulse (recovery time constant: 82 ± 37 sec). APD80 was nonlinearly related to temperature during the RF ablation and responded faster to RF ablation than to purely thermal injury.
Conclusion: The application of RF energy results in significant changes in myocardial cellular electrophysiologic properties. The RF energy bas a combination of thermal and electrotonic effects on the myocardial tissue. The results of this in vitro study may illustrate the cellular basis for commonly observed phenomena in clinical practice. |
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Bibliography: | ark:/67375/WNG-59CQ1V66-M ArticleID:JCE1252 istex:53618A9C28BEA951865CCBD93CD033275543F148 This study was supported in part by Grant 48266 from the National Institutes of Health to Dr. Tung and NSC Grant NSC86‐2314‐B002‐103 to Dr. Wu. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1045-3873 1540-8167 |
DOI: | 10.1111/j.1540-8167.1999.tb00303.x |