rqh1+, a fission yeast gene related to the Bloom's and Werner's syndrome genes, is required for reversible S phase arrest

• Published in: The EMBO journal Vol. 16; no. 10; pp. 2682 - 2692
• Main Authors: Stewart, E, Chapman, C.R, Al-Khodairy, F, Carr, A.M, Enoch, T
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 15.05.1997
• Subjects: Adenosine Triphosphatases - genetics; Amino Acid Sequence; amino acid sequences; Bloom Syndrome - genetics; cell cycle; chromosome segregation; deletions; dna helicase; DNA Helicases - genetics; DNA Replication; enzymes; Exodeoxyribonucleases; genbank/y09426; Gene Deletion; Genes, Fungal; genetic recombination; hus2; Hydroxyurea - pharmacology; interphase; Mitosis - drug effects; Mitosis - genetics; Mitosis - radiation effects; Molecular Sequence Data; Multigene Family; mutants; nucleotide sequences; prevention; recombination; Recombination, Genetic; RecQ DNA helicase; RecQ Helicases; rqh1+ gene; S Phase - genetics; Saccharomyces cerevisiae; Schizosaccharomyces - genetics; Schizosaccharomyces pombe; Schizosaccharomyces pombe Proteins; Sequence Homology, Amino Acid; structural genes; Ultraviolet Rays; Werner Syndrome - genetics; Werner Syndrome Helicase
• ISSN: 0261-4189; 1460-2075; 1460-2075
• DOI: 10.1093/emboj/16.10.2682

In eukaryotic cells, S phase can be reversibly arrested by drugs that inhibit DNA synthesis or DNA damage. Here we show that recovery from such treatments is under genetic control and is defective in fission yeast rqh1 mutants. rqh1(+), previously known as hus(2+), encodes a putative DNA helicase related to the Escherichia coli RecQ helicase, with particular homology to the gene products of the human BLM and WRN genes and the Saccharomyces cerevisiae SGS1 gene. BLM and WRN are mutated in patients with Bloom's syndrome and Werner's syndrome respectively. Both syndromes are associated with genomic instability and cancer susceptibility. We show that, like BLM and SGS1, rqh1(+) is required to prevent recombination and that in fission yeast suppression of inappropriate recombination is essential for reversible S phase arrest.