Quantitative proteome profiles help reveal efficient xylose utilization mechanisms in solventogenic Clostridium sp. strain BOH3

• Published in: Biotechnology and bioengineering Vol. 114; no. 9; pp. 1959 - 1969
• Main Authors: Basu, Anindya, Xin, Fengxue, Lim, Teck Kwang, Lin, Qingsong, Yang, Kun‐Lin, He, Jianzhong
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2017
• Subjects: Alcohols - metabolism; Bacteria; Bacterial Proteins - metabolism; biofuels; BOH3; Butanol; Chemicals; Clostridium; Clostridium - classification; Clostridium - genetics; Clostridium - metabolism; Enzymes; Gene Expression Profiling - methods; Glucose; iTRAQ; Lignocellulose; Metabolic Clearance Rate; Metabolic engineering; Metabolic Flux Analysis - methods; Metabolic Networks and Pathways - physiology; Metabolism; Proteins; Proteome - metabolism; proteome dynamics; Proteomics; Riboflavin; Solvents; Solvents - metabolism; Species Specificity; Sporulation; Strain; Sugar; Sustainable development; Vitamin B; Xylose; Xylose - metabolism; Clostridium; iTRAQ; biofuels; proteome dynamics; BOH3
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.26332

ABSTRACT Development of sustainable biobutanol production platforms from lignocellulosic materials is impeded by inefficient five carbon sugar uptake by solventogenic bacteria. The recently isolated Clostridium sp. strain BOH3 is particularly advantaged in this regard as it serves as a model organism which can simultaneously utilize both glucose and xylose for high butanol (>15 g/L) production. Strain BOH3 was, therefore, investigated for its metabolic mechanisms for efficient five carbon sugar uptake using a quantitative proteomics based approach. The proteomics data show that proteins within the CAC1341‐1349 operon play a pivotal role for efficient xylose uptake within the cells to produce butanol. Furthermore, up‐regulation of key enzymes within the riboflavin synthesis pathway explained that xylose could induce higher riboflavin production capability of the bacteria (e.g., ∼80 mg/L from glucose vs. ∼120 mg/L from xylose). Overall results from the present experimental approach indicated that xylose‐fed BOH3 cultures are subjected to high levels of redox stress which coupled with the solvent stress—trigger a sporulation response within the cells earlier than the glucose‐fed cultures. The study lays the platform for metabolic engineering strategies in designing organisms for efficient butanol and other value‐added chemicals such as riboflavin production. Biotechnol. Bioeng. 2017;114: 1959–1969. © 2017 Wiley Periodicals, Inc. A solventogenic Clostridium sp. strain BOH3, capable of utilizing both five and six carbon sugars, was metabolically investigated in this work using an iTRAQTM based proteomics approach, to reveal the machineries involved in efficient carbon utilization with simultaneous butanol and riboflavin production. The metabolic information revealed in the study would help scientists establish better solventogenic processes for scalable butanol production.