Skin regeneration stimulation: the role of PCL‐platelet gel nanofibrous scaffold

• Published in: Microscopy research and technique Vol. 80; no. 5; pp. 495 - 503
• Main Authors: Ranjbarvan, Parviz, Soleimani, Masoud, Samadi Kuchaksaraei, Ali, Ai, Jafar, Faridi Majidi, Reza, Verdi, Javad
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2017
• Subjects: Biocompatible Materials; Cell adhesion & migration; Cell Differentiation - physiology; Cell Proliferation; Cell Survival; Cells, Cultured - ultrastructure; Epidermis - physiology; Epidermis - ultrastructure; Gels - chemistry; Humans; Immunohistochemistry; Keratin; Keratin-10 - genetics; Keratin-14 - genetics; keratinocyte; Keratinocytes - physiology; Keratinocytes - ultrastructure; Keratins; Microscopy; Microscopy, Electron, Scanning; Nanofibers; Nanofibers - chemistry; Nanofibers - ultrastructure; Nanostructure; platelet rich plasma; Platelets; poly (caprolactone); Polyesters - chemistry; Protein Precursors - genetics; Regeneration; Scaffolds; Skin; Skin (anatomy); Skin Physiological Phenomena; Spectroscopy, Fourier Transform Infrared; Surface Properties; Tensile Strength; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Wound Healing; skin; poly (caprolactone); keratinocyte; platelet rich plasma
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.22821

Skin is the largest organ of the human body. Thus far, tissue engineering of skin has developed rapidly and has used many types of growth factors and nanofibrous scaffolds. In this study, we differentiated neonate keratinocytes for epithelialization on the polycaprolactone‐Platelet gel (PCL‐PG) scaffold. Fabricated PCL nanofibers prepared by electrospinning technology and coated by platelet gel. Subsequently, the structure of the scaffold was evaluated by SEM, FTIR‐ATR, contact angle and tensile test assays. After seeding the neonate keratinocytes on neat PCL and PCL‐PG scaffolds, the epidermal maturation was tested by detecting cytokeratin 10 and loricrin determinants by immunocytochemistry; moreover, keratinocyte genes such as keratin 14, keratin 10, and Involucrin were investigated by real‐time PCR. The results of MTT assay indicated an increase in cell viability and cell proliferation of neonate keratinocytes on PCL‐PG nanofiber scaffolds compared with PCL. RT‐PCR and immunocytochemical analysis showed better cell differentiation on the PCL‐PG scaffolds than neat PCL. Furthermore, SEM microscopy images demonstrated that neo‐keratinocytes enhance adhesion and proliferation on PCL‐PG nanofiber scaffolds. We found that PG increases biocompatibility and wettability of scaffold, cell adhesion, and expression of keratinocyte markers. Overall, this procedure is recommended to be employed in skin tissue engineering and wounds healing. This study introduced PCL‐Platelet gel nanofibrous scaffold and keratinocytes for skin substitution which is a novel method for coating platelet gel on scaffolds. We propose that this construct should be utilized in skin tissue engineering.