Techniques for chromosomal integration and expression optimization in Escherichia coli

• Published in: Biotechnology and bioengineering Vol. 115; no. 10; pp. 2467 - 2478
• Main Authors: Ou, Bingming, Garcia, Carolina, Wang, Yejun, Zhang, Weiping, Zhu, Guoqiang
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2018
• Subjects: Bacteria; chromosomal expression; chromosomal integration; Chromosomes; E coli; Escherichia coli; Escherichia coli (E. coli); expression cassette design; Gene expression; Integration; Metabolic engineering; Metabolism; Optimization; Proteins; synthetic biology; synthetic biology; chromosomal expression; optimization; Escherichia coli (E. coli); chromosomal integration; expression cassette design
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.26790

Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins. Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications.