Genomic and EST microsatellites for Rhododendron aureum (Ericaceae) and cross‐amplification in other congeneric species

• Premise of the study: Our objective of this study was to develop genomic and expressed sequence tag (EST)–derived microsatellites for the endangered species Rhododendron aureum and access their transferability in R. dauricum and R. brachycarpum. • Methods and Results: Twelve genomic microsatellite...

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Published inAmerican journal of botany Vol. 98; no. 9; pp. e250 - e252
Main Authors Li, Lin‐Feng, Yin, Dong‐Xu, Song, Nan, Tang, En‐Hua, Xiao, Hong‐Xing
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.09.2011
Botanical Society of America, Inc
Subjects
Base Sequence
Botany
DNA Primers
Endangered & extinct species
Ericaceae
Ericaceae - genetics
EST
Expressed Sequence Tags
Flowers & plants
Genes, Plant
Genetic diversity
Genomics
heterozygosity
microsatellite
Microsatellite Repeats - genetics
Rhododendron
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Summary:• Premise of the study: Our objective of this study was to develop genomic and expressed sequence tag (EST)–derived microsatellites for the endangered species Rhododendron aureum and access their transferability in R. dauricum and R. brachycarpum. • Methods and Results: Twelve genomic microsatellites were isolated in R. aureum using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol and seven EST‐derived microsatellites were characterized by screening the Rhododendron dbEST database of GenBank. The number of alleles per locus ranged from one to eight. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.00 to 0.73, respectively. • Conclusions: A total of 19 microsatellite loci were developed for R. aureum. Sixteen and 10 of these loci were successfully amplified in R. brachycarpum and R. dauricum, respectively. These microsatellite markers will have potential applications in genetic diversity and conservation genetics studies.
Bibliography:The authors thank Dr. Ming‐Zhou Sun for help with collection of the materials. This work was financially supported by the Fundamental Research Funds for the Central Universities (10QNJJ011) and Jilin Provincial Natural Science Foundation of China (201115004).
These authors contributed equally to this work.
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100153