In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants
Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an in vitro rapid regeneration method using leaf explants of Coleus forskohlii th...
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Published in | Physiology and molecular biology of plants Vol. 16; no. 2; pp. 195 - 200 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
India
Springer-Verlag
01.04.2010
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Subjects | |
Online Access | Get full text |
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Summary: | Coleus forskohlii
is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an
in vitro
rapid regeneration method using leaf explants of
Coleus forskohlii
through direct organogenesis. Leaf explants that were excised into three different segments i.e. proximal (P), middle (M) and distal (D) were cultured on Murashige and Skoog (MS) basal medium supplemented with cytokinins. MS Media containing 5.0 mg L
−1
BAP (6-Benzylaminopurine) promoted regeneration of multiple shoots through direct organogenesis from the leaf, which were further elongated on MS media augmented with 0.1 mg L-1 BAP and 0.1 mg L
−1
IAA (Indole-3-acetic acid), cytokinin and auxin combination. Regenerated and elongated shoots, when transferred to ose resulted in profuse rooting plants that were transferred to soil after acclimatization and maintained in a green house. The current protocol offers a direct, mass propagation method bypassing the callus phase of
C. forskohlii and
is suitable for conservation, large-scale commercial cultivation, and genetic transformation with agronomically desirable traits. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0971-5894 0974-0430 |
DOI: | 10.1007/s12298-010-0021-y |