Phytase-Fe3O4 nanoparticles-loaded microcosms of silica for catalytic remediation of phytate-phosphorous from eutrophic water bodies

• Published in: Environmental science and pollution research international Vol. 26; no. 15; pp. 14988 - 15000
• Main Authors: Dave, Gayatri, Modi, Hasmukh
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2019; Springer Nature B.V
• Subjects: Agricultural practices; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Catalysis; Cell division; Crosslinking; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Eutrophic environments; Eutrophic waters; Eutrophication; Germination; Glutaraldehyde; Iron oxides; Metabolites; Metal-organic frameworks; Microcosms; Nanoparticles; Oxides; Phosphates; Phytase; Phytic acid; Research Article; Seed germination; Seepage; Silica; Silicon dioxide; Synthesis; Toxicity; Transmission electron microscopy; Waste Water Technology; Water Management; Water Pollution Control; Water seepage; Phytase; Phosphate; Phytate
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-019-04794-y

Agriculture P management practices elevate the level of inorganic phosphates in soil that results in phosphorous (P) seepage into water-bodies. This is one of the key factors that have accelerated the menace of eutrophication. Phytic acid (phytate)-P-rich plant metabolite is infamous for its anti-nutrient activity and regularly oozing in to environment though discharge of mono-gastric animals. That has amplified the magnitudes of eutrophication. In this work, for catalysis of phytate-P, the metal-organic framework fabricated towards metal oxides (Fe 3 O 4 ) and phytase in highly ordered microcosms of silica was employed. The synthesized framework was characterized through transmission electron microscopy (TEM) and nitrogen isotherm analysis. Average pore diameter of synthesized bisect oval shaped structures was measured around ≈200 nm. Herein, phytase and Fe 3 O 4 nanoparticles were loaded to the cavities of microcosms through glutaraldehyde-mediated crosslinking. Whereas Fe 3 O 4 nanoparticles act as nano-absorbents that adsorb P liberated from phytase-mediated catalysis of phytate. Kinetic analysis of free and loaded phytase has shown relatively small reduction in catalytic efficiency. These loaded microcosms have removed 60–80% of phytate-phosphate. The optimized process has reduced the growth of photoautotrophs by 50%. Additionally the magnet-assisted separation of loaded microcosms eased the reapplication of loaded microcosms tested for six independent instances. The primary studies conducted to evaluate the geno-toxicity of loaded microcosms have not shown any harmful effect on the process like cell division and seed germination. The efficacy of this method has evaluated towards on-field testing in Changa (Gujarat, India) lake.