Stimulation and inhibition of secretion by phorbol myristate acetate in different cell types

In washed human platelets and in HL60 granulocytes phorbol myristate acetate (PMA, 1 – 2000nM) synergised with threshold concentrations of secretogogues to induce a sustained maximum secretory response. Likewise, superoxide production from HL60 cells maintained a maximal response at PMA concentratio...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 127; no. 3; pp. 950 - 955
Main Authors Blackwell, G.J., Bonser, R.W., Dawson, J., Garland, L.G.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 29.03.1985
Elsevier
Subjects
Animals
Applied sciences
Blood Platelets - drug effects
Blood Platelets - metabolism
Calcimycin - pharmacology
Cell Line
Drug Interactions
Exact sciences and technology
Granulocytes - drug effects
Granulocytes - metabolism
Histamine Release - drug effects
Humans
Lysosomes - enzymology
Mast Cells - drug effects
Mast Cells - metabolism
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Other techniques and industries
Phorbols - pharmacology
Rats
Serotonin - metabolism
Superoxides - metabolism
Tetradecanoylphorbol Acetate - pharmacology
DMSO
U46619
HEPES
fMet-Leu-Phe
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Summary:In washed human platelets and in HL60 granulocytes phorbol myristate acetate (PMA, 1 – 2000nM) synergised with threshold concentrations of secretogogues to induce a sustained maximum secretory response. Likewise, superoxide production from HL60 cells maintained a maximal response at PMA concentrations between 30 – 300nM. At concentrations up to 10nM PMA also augmented calcium ionophore, A23187, stimulated histamine release from rat peritoneal mast cells. However, in the mast cell PMA concentrations above 10nM reduced maximum histamine release in a dose- dependant manner.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(85)80036-X