Serum IL-6, IL-10, and TNFα levels in pediatric sickle cell disease patients during vasoocclusive crisis and steady state condition

• Published in: Cytokine (Philadelphia, Pa.) Vol. 72; no. 1; pp. 43 - 47
• Main Authors: Sarray, Sameh, Saleh, Layal R., Lisa Saldanha, F., Al-Habboubi, Hebah H., Mahdi, Najat, Almawi, Wassim Y.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2015
• Subjects: Adolescent; Adult; Anemia, Sickle Cell - drug therapy; Anemia, Sickle Cell - immunology; Anemia, Sickle Cell - physiopathology; Child; Child, Preschool; Cytokine; Female; Humans; Hydroxyurea - therapeutic use; Infant; Inflammation; Inflammation - blood; Interleukin-10; Interleukin-10 - blood; Interleukin-6 - blood; Male; Retrospective Studies; Sickle cell disease; Tumor Necrosis Factor-alpha - blood; Vascular Diseases - immunology; Vascular Diseases - physiopathology; Vaso-occlusive crisis; Young Adult; Vaso-occlusive crisis; OR; Interleukin-10; SCD; CI; NSAID; Cytokine; Inflammation; VOC; Sickle cell disease; IL-10
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2014.11.030

•We tested the association of reduced IL-10 levels with SCD vaso-occlusive crisis.•IL-10 levels were significantly reduced in VOC than Steady-state SCD patients.•Reduced IL-10 levels correlated with frequency, type, severity, and VOC duration.•We support strong link of reduced IL-10 levels with VOC and its related parameters. Vaso-occlusive crisis (VOC) is a significant complication of sickle cell disease (SCD), and altered production of pro-inflammatory and anti-inflammatory molecules contributed to its pathogenesis. In view of the association of chronic inflammation with VOC onset, and given the capacity of interleukin (IL)-10 as anti-inflammatory, and IL-6, and TNFα as pro-inflammatory cytokines, we tested the association of altered IL-10, IL-6, and TNFα secretion with VOC pathogenesis and its severity. Study subjects comprised 147 SCD patients with active VOC (VOC Group), and 63 pain-free SCD patients for at least 9months before blood collection (Steady-state Group). Serum cytokine concentrations were determined by ELISA. IL-10 levels were significantly reduced, while IL-6 levels were increased in VOC compared to Steady-state groups; serum TNFα levels were comparable between both groups. There was enrichment of low IL-10, but high IL-6 and TNFα quartiles in VOC Group, which translated into increased VOC risk. In contrast, high IL-10, but low IL-6 and TNFα quartiles were seen in Steady-state Group. Correlation analysis demonstrated significant association between reduced IL-10 levels and the frequency, type, severity, and duration of VOC and requirement for hydroxyurea treatment, while IL-6 correlated with duration of VOC episodes. Our data support strong association of reduced IL-10 and increased IL-6 levels with VOC, and their modulation of VOC-related parameters.