Virus Binding and Internalization Assay for Adeno-associated Virus

The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight on virus entry and trafficking. In the following pr...

Full description

Saved in:
Bibliographic Details
Published inBio-protocol Vol. 7; no. 2
Main Authors Berry, Garrett E, Tse, Longping V
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 20.01.2017
Bio-protocol LLC
Subjects
Biology
Methods
Binding
Virus
Adeno-associated virus
Cell surface
Internalization
Virus entry
Online AccessGet full text

Cover

More Information
Summary:The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight on virus entry and trafficking. In the following protocol, we describe a quantitative PCR (qPCR) based method to determine the amount of vector bound to the cell surface and the amount of subsequent virus internalization based on viral genome quantification. This protocol is optimized for studying AAV. Nevertheless, it can serve as a backbone for studying other viruses with careful modification.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2110