Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene

• Published in: Diagnostic microbiology and infectious disease Vol. 37; no. 4; pp. 289 - 291
• Main Authors: Podzorskiab, Raymond P., Baker, Jame’, Merline, Joseph R., Qureshi, Rehana, Holsinger, Joan E.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2000; Elsevier
• Subjects: Biological and medical sciences; DNA Primers; DNA, Viral - analysis; DNA-Directed DNA Polymerase - genetics; Fundamental and applied biological sciences. Psychology; Herpesvirus 1, Human - classification; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - isolation & purification; Herpesvirus 2, Human - classification; Herpesvirus 2, Human - genetics; Herpesvirus 2, Human - isolation & purification; Humans; Microbiology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Techniques used in virology; Virology; Human; Typing; Enzyme; Herpesviridae; Transferases; Alphaherpesvirinae; Genotype; Method; Virus; Polymerase chain reaction; Nucleotidyltransferases; Gene amplification; Gene; Amplicon; Herpesvirus hominis; Clinical isolate; DNA-directed DNA polymerase
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/S0732-8893(00)00153-X

We compared the utility of restriction endonuclease cleavage to type herpes simplex virus DNA polymerase gene amplicons from two well established PCR primer sets. Amplicons typed using Ava II had a 96% correlation to type determined by monoclonal antibody staining, while amplicons typed using Drd I had a 72% correlation to type determined by monoclonal antibody staining.