Development of an ELISA-based kit for the on-farm determination of progesterone in milk

• Published in: Veterinární medicína Vol. 52; no. 1; pp. 19 - 28
• Main Authors: Simersky, R.,Univerzita Palackeho, Olomouc (Czech Republic). Laborator Rustovych Regulatoru, Swaczynova, J.,Univerzita Palackeho, Olomouc (Czech Republic). Laborator Rustovych Regulatoru, Morris, D.A.,Univerzita Palackeho, Olomouc (Czech Republic). Laborator Rustovych Regulatoru, Franek, M.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Strnad, M.,Univerzita Palackeho, Olomouc (Czech Republic). Laborator Rustovych Regulatoru
• Format: Journal Article
• Language: English
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2007; Czech Academy of Agricultural Sciences
• Subjects: Agricultural economics; Antibodies; ANTIGEN ANTIBODY REACTIONS; Binding sites; bovine milk; CICLO ESTRAL; COW MILK; Cow's milk; CYCLE OESTRAL; Dairy farms; Dairy industry; DIAGNOSIS DE LA GESTACION; DIAGNOSTIC DE GESTATION; ELISA; Estrus; Horseradish peroxidase; http://www.fao.org/aos/agrovoc#c_15968; http://www.fao.org/aos/agrovoc#c_16017; http://www.fao.org/aos/agrovoc#c_16080; http://www.fao.org/aos/agrovoc#c_27596; http://www.fao.org/aos/agrovoc#c_4788; http://www.fao.org/aos/agrovoc#c_503; http://www.fao.org/aos/agrovoc#c_5322; http://www.fao.org/aos/agrovoc#c_6218; http://www.fao.org/aos/agrovoc#c_6429; http://www.fao.org/aos/agrovoc#c_8044; LAIT DE VACHE; LECHE DE VACA; MAITRISE DE LA REPRODUCTION; METHODE; METHODS; METODOS; Milk; OESTROUS CYCLE; oestrus; Peroxidase; Polyclonal antibodies; Pregnancy; PREGNANCY DIAGNOSIS; PROGESTERONA; PROGESTERONE; RADIOIMMUNOASSAY; REACCION ANTIGENO ANTICUERPO; REACTION ANTIGENE ANTICORPS; REPRODUCCION DIRIGIDA; reproduction; REPRODUCTION CONTROL; TECHNIQUE RADIOIMMUNOLOGIQUE; TECNICAS RADIOINMUNOLOGICAS; TEST ELISA; ULTRASON; ULTRASONICS; ULTRASONIDO
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/2009-VETMED

We report here the development of a simple ELISA test for the measurement of progesterone levels in bovine milk based on the use of polyclonal antibodies raised against 11alpha-hydroxyprogesterone-hemisuccinate and competition for antibody binding sites between free progesterone and horseradish peroxidase-labelled progesterone. Binding is quantified by means of the colour reaction between horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine. The method is cost effective, sensitive and easy to perform. Our results show that it can be used under farm conditions not only for pregnancy detection, but also for oestrus detection.