Phytohemagglutinin-stimulated calcium signal in lymphocytes of alcoholics before, during and after detoxification

Acute and chronic exposure to ethanol influences intracellular calcium homeostasis via NMDA receptors, direct regulation of calcium channels or the phosphoinositide pathway. To explore the influence of alcohol withdrawal on calcium metabolism, we have investigated the resting and phytohemagglutinin...

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Bibliographic Details
Published inPsychiatry research Vol. 81; no. 2; pp. 157 - 162
Main Authors Bondy, Brigitta, Engel, Rolf R, de Jonge, Sylvia, Schütz, Christian G, Soyka, Michael
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 16.11.1998
Elsevier
Subjects
Adult
Alcohol withdrawal
Alcohol Withdrawal Delirium - immunology
Alcoholism
Alcoholism - immunology
Alcoholism - rehabilitation
Alcoholism and acute alcohol poisoning
Biological and medical sciences
Calcium - blood
Calcium signal
Calcium Signaling - immunology
Female
Homeostasis
Humans
Lymphocyte Activation - immunology
Lymphocytes
Male
Medical sciences
Middle Aged
Phytohemagglutinin
Phytohemagglutinins - immunology
Toxicology
Calcium signal
Phytohemagglutinin
Lymphocytes
Alcoholism
Alcohol withdrawal
Human
Alcoholic beverage
Signal transduction
Treatment
Dependence
Detoxification
Calcium Ions
Metabolism
Lymphocyte
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Summary:Acute and chronic exposure to ethanol influences intracellular calcium homeostasis via NMDA receptors, direct regulation of calcium channels or the phosphoinositide pathway. To explore the influence of alcohol withdrawal on calcium metabolism, we have investigated the resting and phytohemagglutinin (PHA) stimulated [Ca 2+] i in lymphocytes of 10 alcoholics before, during and after withdrawal. Our findings suggest that both compartments of the PHA-stimulated signal are affected in alcoholics, with flattening of the initial peak and sustained calcium influx, as long as severe vegetative signs are present. MANOVA results showed significant interaction effects for both measurement points, for the initial peak, 40 s after stimulation ( P=0.05), and especially for the sustained influx at the end of the observation period ( P=0.001). The detailed mechanisms of this disturbed calcium homeostasis need further investigation.
ISSN:0165-1781
1872-7123
DOI:10.1016/S0165-1781(98)00102-4