Subcellular distribution of hydralazine in rat single vascular muscle cells

High specific activity (20 Ci/mmol) tritiated hydralazine ( 3Hyd) distribution in isolated, cultured vascular muscle cells was determined to identify the sites of Hyd binding. 3Hyd dose-dependently bound to extracellular protein and to the area of organelles which secrete these proteins. Increased e...

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Published inCell biology international reports Vol. 16; no. 10; pp. 1023 - 1039
Main Authors Baker, J.R.J., Hedwall, P.R., Hermsmeyer, K.
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 01.10.1992
San Diego, CA Academic Press
New York, NY
Subjects
Animals
Aorta - cytology
Biological and medical sciences
Cardiovascular system
Cells, Cultured
Hydralazine - analysis
Hydralazine - metabolism
Hydralazine - pharmacology
Medical sciences
Microscopy, Electron
Muscle Contraction - drug effects
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - metabolism
Muscle, Smooth, Vascular - ultrastructure
Pharmacology. Drug treatments
Radioligand Assay
Rats
Vasodilator agent
Rat
Rodentia
Smooth muscle
Binding site
In vitro
Vertebrata
Mammalia
Animal
Blood vessel
Antihypertensive agent
Localization
Mechanism of action
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Summary:High specific activity (20 Ci/mmol) tritiated hydralazine ( 3Hyd) distribution in isolated, cultured vascular muscle cells was determined to identify the sites of Hyd binding. 3Hyd dose-dependently bound to extracellular protein and to the area of organelles which secrete these proteins. Increased extracellular binding after Hyd pre-exposure suggests new binding sites may be exacerbated as a result of Hyd interactions. These experiments suggest a potentially important feature of the mechanism of action of this directly acting vasodilator.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0309-1651
1878-240X
DOI:10.1016/S0309-1651(06)80055-X