On-line liquid chromatography coupled with high field NMR and mass spectrometry (LC-NMR-MS): a new technique for drug metabolite structure elucidation

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 15; no. 12; pp. 1903 - 1912
• Main Authors: Burton, K.I., Everett, J.R., Newman, M.J., Pullen, F.S., Richards, D.S., Swanson, A.G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.1997; Elsevier Science
• Subjects: Acetaminophen - chemistry; Acetaminophen - urine; Analgesics, Non-Narcotic - chemistry; Analgesics, Non-Narcotic - urine; Analysis; Biological and medical sciences; Chromatography; Chromatography, Liquid; General pharmacology; Human urine; Humans; Magnetic Resonance Spectroscopy - methods; Male; Mass spectrometry; Mass Spectrometry - methods; Medical sciences; Molecular Structure; NMR; Online Systems; Paracetamol; Pharmacology. Drug treatments; Reference Values; NMR; Paracetamol; Mass spectrometry; Human urine; Chromatography; Human; Urine; Biological fluid; Metabolite; Antimigrainous agent; Liquid chromatography; NMR spectrometry; Analgesic; Ultraviolet detector; Antipyretic; Structural analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(96)02034-1

High performance liquid chromatography has been coupled simultaneously to high field NMR and MS detectors, giving UV, NMR and mass spectra for each component in a mixture, after on-line separation. This powerful new tool for the structure elucidation of components in mixtures without isolation has been successfully applied to the analysis of the metabolites of paracetamol in human urine.