Female-enriched expression of ERα during gonad differentiation of the urodele amphibian Pleurodeles waltl

• Published in: General and comparative endocrinology Vol. 156; no. 2; pp. 234 - 245
• Main Authors: Ko, Chia-I, Chesnel, Amand, Mazerbourg, Sabine, Kuntz, Sandra, Flament, Stéphane, Chardard, Dominique
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.04.2008
• Subjects: Amphibian; Aromatase; Endocrinology & Metabolism; Estrogen receptor; Freshwater; Pleurodeles waltl; Sex differentiation; Estrogen receptor; Sex differentiation; Aromatase; Amphibian
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2008.01.012

Abstract In the amphibian Pleurodeles waltl , estradiol treatment of genetically male larvae (ZZ) induces male-to-female sex reversal whereas heat treatment of genetically female larvae (ZW) inhibits estradiol synthesis and leads to female-to-male sex reversal. No data are available on estrogen receptors in this species. In the present study, we have isolated a unique full-length pw ERα cDNA and its 5′-flanking region whose promoter activity was confirmed by transfection assays. RT-PCR studies performed in adult animals using ERα-specific primers, revealed that pw ERα mRNA was present mainly in reproductive tissues: gonads, fat body and oviduct. Pw ERα transcript was also detected in liver, suggesting its implication in vitellogenesis control as in numerous oviparous species. The level of pw ERα transcripts was also studied during gonad differentiation by quantitative real-time PCR. At stage 5430d pw ERα expression in gonads of ZW larvae was significantly higher than in ZZ ones. This sex-specific discrimination was confirmed when gonad–mesonephros–interrenal complexes (GMI), taken at the same stage, were subjected to whole mount in situ hybridization. In comparison, the female-enriched expression of P450 aromatase, which was studied as a control of ovary differentiation, was observed earlier (stage 54). In ZW larvae reared at 32 °C, a condition leading to sex reversal, pw ERα mRNA level at stage 5430d was lower than in control females. Taken together, these results showing a female-enriched and thermosensitive expression of pw ERα suggest an important role for this receptor in gonad differentiation of the urodele amphibian Pleurodeles waltl.