Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40

In the ‘post-genome’ era, reverse genetics is one of the most informative and powerful means to investigate protein function. The chicken B lymphocyte line DT40 is widely used for reverse genetics because the cells have a number of advantages, including efficient gene targeting as well as a remarkab...

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Bibliographic Details
Published inDNA Repair Vol. 3; no. 8; pp. 1175 - 1185
Main Authors Yamazoe, Mitsuyoshi, Sonoda, Eiichiro, Hochegger, Helfrid, Takeda, Shunichi
Format Book Review Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.08.2004
Subjects
Animals
Apoptosis
B-Lymphocytes - pathology
Cell Line
Chickens
Chromosomes - ultrastructure
Conditional mutant
DNA - metabolism
DNA Damage
DNA Repair
DT40
Gallus gallus
Gene disruption
Genes, p53
Genetic Techniques
Genome instability
Homologous DNA recombination
Interphase
Models, Genetic
Mutation
Phenotype
Promoter Regions, Genetic
Recombination, Genetic
Genome instability
Conditional mutant
DT40
Gene disruption
Homologous DNA recombination
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Summary:In the ‘post-genome’ era, reverse genetics is one of the most informative and powerful means to investigate protein function. The chicken B lymphocyte line DT40 is widely used for reverse genetics because the cells have a number of advantages, including efficient gene targeting as well as a remarkably stable phenotype. Furthermore, the absence of functional p53 in DT40 cells enables identification of DNA damage using chromosome analysis by suppressing damage-induced apoptosis during interphase. This review summarizes the contribution of DT40 cells to reverse genetic studies of DNA damage response pathways in higher eukaryotic cells.
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ISSN:1568-7864
1568-7856
DOI:10.1016/j.dnarep.2004.03.039