Detection of nongroup A rotaviruses in faecal samples of pigs in the Czech Republic

• Published in: Veterinární medicína Vol. 54; no. 1; pp. 12 - 18
• Main Authors: Smitalova, R.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Rodak, L.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Smid, B.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Psikal, I.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)
• Format: Journal Article
• Language: English
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2009; Czech Academy of Agricultural Sciences
• Subjects: CZECH REPUBLIC; Diarrhea; ELECTROFORESIS; Electron microscopy; ELECTROPHORESE; ELECTROPHORESIS; ELISA; ENTERITE; ENTERITIS; Enzyme-linked immunosorbent assay; GENE; Gene sequencing; GENES; group b rotavirus; group c rotavirus; http://www.fao.org/aos/agrovoc#c_10625; http://www.fao.org/aos/agrovoc#c_15968; http://www.fao.org/aos/agrovoc#c_2523; http://www.fao.org/aos/agrovoc#c_27583; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_33095; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_34753; http://www.fao.org/aos/agrovoc#c_4810; http://www.fao.org/aos/agrovoc#c_5872; LECHON; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; nongroup a rotavirus; NUCLEOTIDE SEQUENCE; Nucleotides; PCR; PIGLETS; Polymerase chain reaction; PORCELET; PORCINE ROTAVIRUS; REPUBLICA CHECA; REPUBLIQUE TCHEQUE; Rotavirus; ROTAVIRUS PORCINE; ROTAVIRUS PORCINO; rt-pcr, semi-nested pcr; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; Swine; TEST ELISA; Viruses
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/3081-VETMED

Besides group A rotaviruses, group B and C rotaviruses have been detected as the cause of diarrheal diseases in pigs. Out of a set of 329 faecal samples from pigs, 16 samples were selected in which rotavirus was detected by electron microscopy and at the same time group A rotavirus was excluded by the ELISA method. Rotaviruses were assayed using specific primers for detection of group B and C rotaviruses, and RT-PCR and semi-nested PCR methods. In one sample, no rotavirus of group B or C was detected; in the remaining 15 samples rotavirus group C was detected, in two samples together with group B rotavirus. Sequencing of the obtained PCR products and comparison with corresponding gene sequences revealed 80% nucleotide sequence identity between group B rotaviruses and available sequences of porcine isolates. A nucleotide sequence identity of 92% was obtained in group C rotaviruses as compared with the Cowden strain.