Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea

Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this...

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Published inBio-protocol Vol. 7; no. 13; p. e2372
Main Authors Salbitani, Giovanna, Bottone, Claudia, Carfagna, Simona
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.07.2017
Bio-protocol LLC
Subjects
Biology
Methods
Galdieria phlegrea
Redox state
Microalgae
Glutathione reductase
Glutathione
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Summary:Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga .
Bibliography:ObjectType-Article-1
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2372