Extreme dryness and DNA-protein cross-links

Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered...

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Bibliographic Details
Published inAdvances in space research Vol. 12; no. 4; p. 265
Main Authors Bieger-Dose, A, Dose, K, Meffert, R, Mehler, M, Risi, S
Format Journal Article
LanguageEnglish
Published England 1992
Subjects
Aspergillus ochraceus - genetics
Aspergillus ochraceus - metabolism
Autoradiography
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
Bacterial Proteins - metabolism
Desiccation
DNA Damage
DNA, Bacterial - isolation & purification
DNA, Bacterial - metabolism
Electrophoresis, Agar Gel
Spores, Bacterial
Vacuum
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Summary:Exposure of fungal conidia (Aspergillus ochraceus) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125 J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32P by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.
ISSN:0273-1177
DOI:10.1016/0273-1177(92)90181-V