Structures of poly(dA-dT,ip5dU) containing various small amounts of the antiherpetic 5-isopropyl-2′-deoxyuridine

• Published in: Biochemical and biophysical research communications Vol. 185; no. 1; pp. 96 - 102
• Main Authors: Sági, János, Štokrová, Jitka, Vorlíčková, Michaela, Spánová, Alena, Kypr, Jaroslav, Ruff, Éva, Ötvös, László
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 29.05.1992; Elsevier
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Antiviral Agents - chemistry; Antiviral Agents - therapeutic use; Biological and medical sciences; Circular Dichroism; Deoxyuridine - analogs & derivatives; Deoxyuridine - chemistry; Deoxyuridine - therapeutic use; Herpesviridae Infections - drug therapy; Medical sciences; Microscopy, Electron; Nucleic Acid Conformation; Pharmacology. Drug treatments; Poly dA-dT - chemistry; Polydeoxyribonucleotides - chemistry; Polydeoxyribonucleotides - therapeutic use; Spectrophotometry, Ultraviolet; electron microscopy; circular dichroism; 5-isopropyl-2′-deoxyuridine; ultraviolet; is abbreviated as ip5dU. The ip5 is also used in ip5dUMP, ip5dUTP and the copolymer poly(dA-dT,ip5dU). UV, CD and EM stand for; Infection; Viral disease; Analog; DNA; Herpes; Antiviral; Polydeoxyribonucleotide; Thymidine; Mechanism of action; In vitro; Pyrimidine nucleotide; Induced conformation
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(05)80960-X

Three different concentrations of the antiherpetic agent 5-isopropyl-2′-deoxyuridine (ip5dU) were introduced into the synthetic DNA poly(dA-dT) to analyze resulting copolymers by electron microscopy, UV absorption and CD spectroscopy. The poly(dA-dT,ip5dU) containing 1.3 and 4.3% ip5dU did not much differ from the parent poly(dA-dT) but poly(dA-dT,ip5dU) with 7.1% ip5dU behaved in an unusual way. Results are explained by the notion that if bulky isopropyls occur sufficiently close to each other then stable hairpins protruding from the double helix are formed, presumably to accomodate the ip5dU-s into the loops.